Merrett T G, Pantin C F, Dimond A H, Merrett J
Allergy. 1980 Sep;35(6):491-501. doi: 10.1111/j.1398-9995.1980.tb01796.x.
Sera from 425 patients were analysed with Phadebas RAST reagents against a panel of 19 allergens and the most commonly positive results were to grass pollen, Dermatophagoides pteronyssinus and cat epithelium. Of the 275 sera RAST-positive to any allergen, 261 were positive by a RAST technique which used a mixture of the three most common U.K. allergens; the remainder were usually positive to a mould. We conclude that 1) when a positive result is obtained after screening by the mixed-allergen RAST and a representative mould RAST, then other positive RAST results are possible, but that the extent of the investigation can be guided by a knowledge of the total serum IgE level and a computer-based history analysis; 2) when a mixed-allergen RAST and a representative mould RAST is negative, then a more specified RAST including all 19 allergens used in this investigation will be negative; 3) a positive mixed-allergen RAST result is likely to be associated with a total serum IgE level over 40 U/ml and a positive symptom pattern score derived from the computer-based history analysis.
使用Phadebas RAST试剂对425名患者的血清进行了分析，检测了一组19种变应原，最常见的阳性结果是对草花粉、屋尘螨和猫上皮细胞呈阳性。在对任何变应原呈RAST阳性的275份血清中，261份通过使用三种最常见的英国变应原混合物的RAST技术呈阳性；其余的通常对霉菌呈阳性。我们得出以下结论：1）当通过混合变应原RAST和代表性霉菌RAST筛查后获得阳性结果时，其他RAST阳性结果是可能的，但调查范围可根据血清总IgE水平的知识和基于计算机的病史分析来指导；2）当混合变应原RAST和代表性霉菌RAST为阴性时，包括本研究中使用的所有19种变应原的更特定的RAST将为阴性；3）混合变应原RAST阳性结果可能与血清总IgE水平超过40 U/ml以及基于计算机的病史分析得出的阳性症状模式评分相关。
