Bibikov M V, Selekhova G N, Vostrov S N, Ivanitskaia L P, Fishman V M
Antibiotiki. 1980 Dec;25(12):899-904.
An optimal fermentation medium for detection of antibiotic-producing cultures among Micromonosporaceae at the stage of primary screening was developed. The method of the experiment factor design with regard both for the qualitative and the quantitative variation of the medium composition was used. The number of the active strains detected with the use of medium M1 amounts to 76.4 per cent of the cultures tested. The same efficiency was observed with simultaneous use of 6 different fermentation media. The use of the new medium significantly decreases the labour-consuming character of the procedure.
开发了一种用于在初筛阶段检测小单孢菌科中产抗生素培养物的最佳发酵培养基。采用了针对培养基成分的定性和定量变化的实验因子设计方法。使用培养基M1检测到的活性菌株数量占所测试培养物的76.4%。同时使用6种不同的发酵培养基时也观察到了相同的效率。新培养基的使用显著降低了该程序的劳动强度。
