Starzyk R M, Merritt R B
Biochem Genet. 1980 Aug;18(7-8):755-64. doi: 10.1007/BF00484591.
The interspecies homology of dace supernatant (A2,AB,B2) and mitochondrial (C2) malate dehydrogenase isozymes has been established through cell fractionation and tissue distribution studies. Isolated supernatant malate dehydrogenase (s-MDH) isozymes show significant differences in Michaelis constants for oxaloacetate and in pH optima. Shifts in s-MDH isozyme pH optima with temperature may result in immediate compensation for increase in ectotherm body pH with decrease in temperature, but duplicate s-MDH isozymes are probably maintained through selection for tissue specific regulation of metabolism.
通过细胞分级分离和组织分布研究，已确定了雅罗鱼属的上清液（A2、AB、B2）和线粒体（C2）苹果酸脱氢酶同工酶的种间同源性。分离出的上清液苹果酸脱氢酶（s-MDH）同工酶在草酰乙酸的米氏常数和最适pH值方面存在显著差异。s-MDH同工酶最适pH值随温度的变化可能会立即补偿变温动物体温下降时体温pH值的升高，但重复的s-MDH同工酶可能是通过对组织特异性代谢调节的选择而得以维持的。
