配体形成是大鼠肝细胞培养中细胞色素P - 450维持机制的证据。美替拉酮具有强大的维持作用。
Evidence that ligand formation is a mechanism underlying the maintenance of cytochrome P-450 in rat liver cell culture. Potent maintenance by metyrapone.
作者信息
Paine A J, Villa P, Hockin L J
出版信息
Biochem J. 1980 Jun 15;188(3):937-9. doi: 10.1042/bj1880937.
Abstract
The loss of cytochrome P-450 in cultured rat hepatocytes can be prevented by substituted pyridines, especially isonicotinamide, 3-hydroxypyridine and metyrapone. The effect of these compounds is independent of protein synthesis, suggesting that they maintain pre-existing cytochrome P-450. The efficiency of pyridines at maintaining cytochrome P-450 in hepatocyte culture is highly correlated with their ability to bind to this cytochrome, suggesting that ligand formation with cytochrome P-450 prevents its accelerated turnover in liver cell culture.
摘要
培养的大鼠肝细胞中细胞色素P - 450的丧失可被取代吡啶，尤其是异烟酰胺、3 - 羟基吡啶和甲吡酮所阻止。这些化合物的作用与蛋白质合成无关，这表明它们维持已有的细胞色素P - 450。吡啶在肝细胞培养中维持细胞色素P - 450的效率与其与该细胞色素结合的能力高度相关，这表明与细胞色素P - 450形成配体可防止其在肝细胞培养中加速周转。
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