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1
Evidence that ligand formation is a mechanism underlying the maintenance of cytochrome P-450 in rat liver cell culture. Potent maintenance by metyrapone.配体形成是大鼠肝细胞培养中细胞色素P - 450维持机制的证据。美替拉酮具有强大的维持作用。
Biochem J. 1980 Jun 15;188(3):937-9. doi: 10.1042/bj1880937.
2
The role of 5-aminolaevulinate synthase, haem oxygenase and ligand formation in the mechanism of maintenance of cytochrome P-450 concentration in hepatocyte culture.5-氨基乙酰丙酸合成酶、血红素加氧酶及配体形成在维持肝细胞培养物中细胞色素P-450浓度机制中的作用
Biochem J. 1983 Mar 15;210(3):855-7. doi: 10.1042/bj2100855.
3
The substituted pyridines metyrapone and nicotinamide are inducers of 5-aminolevulinate synthase and cytochrome P-450 in hepatocyte culture.取代吡啶美替拉酮和烟酰胺是肝细胞培养中5-氨基酮戊酸合酶和细胞色素P-450的诱导剂。
Biochem Pharmacol. 1982 May 1;31(9):1735-41. doi: 10.1016/0006-2952(82)90677-3.
4
Immunochemical quantification of cytochrome P450IA and IIB subfamilies in the livers of metyrapone-treated rats. Relevance to the ability of metyrapone to prevent the loss of cytochrome P-450 in rat hepatocyte culture.甲吡酮处理大鼠肝脏中细胞色素P450IA和IIB亚家族的免疫化学定量分析。与甲吡酮预防大鼠肝细胞培养中细胞色素P-450丢失能力的相关性。
Biochem J. 1990 May 1;267(3):715-9. doi: 10.1042/bj2670715.
5
Maintenance of cytochrome P-450IA2, IIB1 and IIB2 mRNAs by metyrapone in rat hepatocyte culture.美替拉酮对大鼠肝细胞培养物中细胞色素P-450IA2、IIB1和IIB2 mRNA的维持作用
Biochem Soc Trans. 1990 Dec;18(6):1203. doi: 10.1042/bst0181203.
6
Glucocorticoid receptor-independent transcriptional induction of cytochrome P450 3A1 by metyrapone and its potentiation by glucocorticoid.美替拉酮对细胞色素P450 3A1的糖皮质激素受体非依赖性转录诱导作用及其被糖皮质激素增强的作用。
Mol Pharmacol. 1996 Oct;50(4):856-63.
7
Effect of the adrenal 11-beta-hydroxylase inhibitor metyrapone on human hepatic cytochrome P-450 expression: induction of cytochrome P-450 3A4.肾上腺11-β-羟化酶抑制剂美替拉酮对人肝细胞色素P-450表达的影响：细胞色素P-450 3A4的诱导作用
Drug Metab Dispos. 2000 Jan;28(1):96-101.
8
The maintenance of cytochrome P-450 in rat hepatocyte culture: some applications of liver cell cultures to the study of drug metabolism, toxicity and the induction of the P-450 system.大鼠肝细胞培养中细胞色素P-450的维持：肝细胞培养在药物代谢、毒性及P-450系统诱导研究中的一些应用。
Chem Biol Interact. 1990;74(1-2):1-31. doi: 10.1016/0009-2797(90)90055-r.
9
The relationship between the ability of pyridine and substituted pyridines to maintain cytochrome P-450 and inhibit protein synthesis in rat hepatocyte cultures.吡啶及取代吡啶在大鼠肝细胞培养物中维持细胞色素P - 450的能力与抑制蛋白质合成之间的关系。
Biochem Pharmacol. 1980 Jun 15;29(12):1773-7. doi: 10.1016/0006-2952(80)90139-2.
10
Relationship between the ability of nicotinamide to maintain nicotinamide-adenine dinucleotide in rat liver cell culture and its effect on cytochrome P-450.烟酰胺在大鼠肝细胞培养物中维持烟酰胺腺嘌呤二核苷酸的能力与其对细胞色素P-450的影响之间的关系
Biochem J. 1979 Nov 15;184(2):461-3. doi: 10.1042/bj1840461.

引用本文的文献

1
The role of 5-aminolaevulinate synthase, haem oxygenase and ligand formation in the mechanism of maintenance of cytochrome P-450 concentration in hepatocyte culture.5-氨基乙酰丙酸合成酶、血红素加氧酶及配体形成在维持肝细胞培养物中细胞色素P-450浓度机制中的作用
Biochem J. 1983 Mar 15;210(3):855-7. doi: 10.1042/bj2100855.
2
Alveolar type II and Clara cells: isolation and xenobiotic metabolism.II型肺泡细胞和克拉拉细胞：分离与外源性物质代谢
Environ Health Perspect. 1984 Jun;56:95-101. doi: 10.1289/ehp.845695.
3
Cytochrome P-450 monooxygenase, epoxide hydrolase and flavin monooxygenase activities in Clara cells and alveolar type II cells isolated from rabbit.从兔分离的克拉拉细胞和II型肺泡细胞中的细胞色素P-450单加氧酶、环氧化物水解酶和黄素单加氧酶活性。
Cell Biol Toxicol. 1985 Jan;1(2):57-65. doi: 10.1007/BF00717791.
4
Immunochemical quantification of cytochrome P450IA and IIB subfamilies in the livers of metyrapone-treated rats. Relevance to the ability of metyrapone to prevent the loss of cytochrome P-450 in rat hepatocyte culture.甲吡酮处理大鼠肝脏中细胞色素P450IA和IIB亚家族的免疫化学定量分析。与甲吡酮预防大鼠肝细胞培养中细胞色素P-450丢失能力的相关性。
Biochem J. 1990 May 1;267(3):715-9. doi: 10.1042/bj2670715.
5
Co-cultures of hepatocytes with epithelial-like cell lines: expression of drug-biotransformation activities by hepatocytes.肝细胞与上皮样细胞系的共培养：肝细胞药物生物转化活性的表达
Cell Biol Toxicol. 1991 Jan;7(1):1-14. doi: 10.1007/BF00121326.
6
Maintenance of total cytochrome P-450 content in rat hepatocyte culture and the abundance of CYP1A2 and CYP2B1/2 mRNAs.大鼠肝细胞培养物中总细胞色素P-450含量的维持以及CYP1A2和CYP2B1/2 mRNA的丰度
Biochem J. 1992 Aug 1;285 ( Pt 3)(Pt 3):929-32. doi: 10.1042/bj2850929.

本文引用的文献

1
Ligand interactions with cytochrome P-450. I. Binding of primary amines.
Biochemistry. 1969 Aug;8(8):3455-63. doi: 10.1021/bi00836a049.
2
Metyrapone interaction with hepatic microsomal cytochrome P-450 from rats treated with phenobarbital.甲吡酮与经苯巴比妥处理的大鼠肝脏微粒体细胞色素P-450的相互作用。
Biochem Biophys Res Commun. 1969 Oct 22;37(3):477-85. doi: 10.1016/0006-291x(69)90940-1.
3
Binding of nitrogen containing compounds to microsomal cytochromes.含氮化合物与微粒体细胞色素的结合。
Xenobiotica. 1971 Jul-Oct;1(4):507-20. doi: 10.3109/00498257109041519.
4
The use of new methods to measure: the effect of diet and inducers of microsomal enzyme synthesis on cytochrome P-450 in liver homogenates, and on metabolism of dimethyl nitrosamine.采用新方法测量：饮食及微粒体酶合成诱导剂对肝匀浆中细胞色素P - 450以及对二甲基亚硝胺代谢的影响。
Biochem Pharmacol. 1974 Apr 1;23(7):1173-80. doi: 10.1016/0006-2952(74)90291-3.
5
Spectral studies of drug interaction with hepatic microsomal cytochrome.药物与肝微粒体细胞色素相互作用的光谱研究
Mol Pharmacol. 1967 Mar;3(2):113-23.
6
Apparent lack of correlation between the loss of cytochrome P-450 in hepatic parenchymal cell culture and the stimulation of haem oxygenase activity.肝实质细胞培养中细胞色素P-450的丧失与血红素加氧酶活性的刺激之间明显缺乏相关性。
Biochem Biophys Res Commun. 1978 Mar 30;81(2):672-9. doi: 10.1016/0006-291x(78)91589-9.
7
Inhibition by cycloheximide of degradation of cytochrome P-450 in primary cultures of adult rat liver parenchymal cells and in vivo.放线菌酮对成年大鼠肝实质细胞原代培养物及体内细胞色素P-450降解的抑制作用。
Biochem J. 1979 Jun 15;180(3):621-30. doi: 10.1042/bj1800621.
8
Drug metabolism in adult rat hepatocytes in primary monolayer culture.原代单层培养的成年大鼠肝细胞中的药物代谢
Gastroenterology. 1977 Jun;72(6):1232-9.
9
Relationship between the ability of nicotinamide to maintain nicotinamide-adenine dinucleotide in rat liver cell culture and its effect on cytochrome P-450.烟酰胺在大鼠肝细胞培养物中维持烟酰胺腺嘌呤二核苷酸的能力与其对细胞色素P-450的影响之间的关系
Biochem J. 1979 Nov 15;184(2):461-3. doi: 10.1042/bj1840461.

配体形成是大鼠肝细胞培养中细胞色素P - 450维持机制的证据。美替拉酮具有强大的维持作用。

Evidence that ligand formation is a mechanism underlying the maintenance of cytochrome P-450 in rat liver cell culture. Potent maintenance by metyrapone.

作者信息

Paine A J, Villa P, Hockin L J

出版信息

Biochem J. 1980 Jun 15;188(3):937-9. doi: 10.1042/bj1880937.

DOI:10.1042/bj1880937
PMID:7470047
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1161982/
Abstract

The loss of cytochrome P-450 in cultured rat hepatocytes can be prevented by substituted pyridines, especially isonicotinamide, 3-hydroxypyridine and metyrapone. The effect of these compounds is independent of protein synthesis, suggesting that they maintain pre-existing cytochrome P-450. The efficiency of pyridines at maintaining cytochrome P-450 in hepatocyte culture is highly correlated with their ability to bind to this cytochrome, suggesting that ligand formation with cytochrome P-450 prevents its accelerated turnover in liver cell culture.

摘要

培养的大鼠肝细胞中细胞色素P - 450的丧失可被取代吡啶，尤其是异烟酰胺、3 - 羟基吡啶和甲吡酮所阻止。这些化合物的作用与蛋白质合成无关，这表明它们维持已有的细胞色素P - 450。吡啶在肝细胞培养中维持细胞色素P - 450的效率与其与该细胞色素结合的能力高度相关，这表明与细胞色素P - 450形成配体可防止其在肝细胞培养中加速周转。