烟酰胺在大鼠肝细胞培养物中维持烟酰胺腺嘌呤二核苷酸的能力与其对细胞色素P-450的影响之间的关系
Relationship between the ability of nicotinamide to maintain nicotinamide-adenine dinucleotide in rat liver cell culture and its effect on cytochrome P-450.
作者信息
Paine A J, Hockin L J, Legg R F
出版信息
Biochem J. 1979 Nov 15;184(2):461-3. doi: 10.1042/bj1840461.
Abstract
Rat hepatocytes cultured for 24 h lose 60% of their NAD content. Treatment with nicotinamide prevents the loss of NAD as well as the previously reported loss of cytochrome P-450, suggesting a possible causal relationship. However, isonicotinamide also prevents the loss of cytochrome P-450, but does not increase the concentration of NAD, demonstrating that the ability of nicotinamide to maintain cytochrome P-450 is not apparently related to its effect on the NAD content of cultured hepatocytes.
摘要
培养24小时的大鼠肝细胞失去其60%的烟酰胺腺嘌呤二核苷酸（NAD）含量。用烟酰胺处理可防止NAD的损失以及先前报道的细胞色素P - 450的损失，这表明可能存在因果关系。然而，异烟酰胺也能防止细胞色素P - 450的损失，但不会增加NAD的浓度，这表明烟酰胺维持细胞色素P - 450的能力显然与其对培养肝细胞NAD含量的影响无关。
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Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
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