将氨基酸掺入营养不良仓鼠的可溶性和膜蛋白组分中。
Incorporation of amino acids into soluble and membrane protein fractions of dystrophic hamsters.
作者信息
Nicholls D M, Creasy R C, Chin-See M W, Carlisle J A, Lange A B, Saleem M
出版信息
Biochem J. 1980 Aug 15;190(2):341-8. doi: 10.1042/bj1900341.
The incorporation of labelled leucine was measured in protein fractions of muscle in intact control and dystrophic female hamsters and also in cell-free preparations obtained from these animals. The labelling of the soluble sarcoplasmic protein fraction, the microsomal protein fraction and the sarcolemma protein fraction was increased in the dystrophic hindleg muscle. The specific radioactivities of the sarcolemma protein fraction and other fractions were increased markedly relative to that of free leucine in the dystrophic muscle. In cell-free preparations where ribonuclease effects were avoided, the dystrophic muscle exhibited an increased synthesis of peptide bonds.
在完整的对照雌性仓鼠和营养不良雌性仓鼠的肌肉蛋白质组分中，以及从这些动物获得的无细胞制剂中，测量了标记亮氨酸的掺入情况。在营养不良的后肢肌肉中，可溶性肌浆蛋白组分、微粒体蛋白组分和肌膜蛋白组分的标记增加。相对于营养不良肌肉中游离亮氨酸的比放射性，肌膜蛋白组分和其他组分的比放射性显著增加。在避免核糖核酸酶作用的无细胞制剂中，营养不良的肌肉表现出肽键合成增加。
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