Wrogemann K, Quilliam N M, Nylen E G, Johnson M D
Can J Biochem. 1977 Aug;55(8):894-900. doi: 10.1139/o77-131.
In liver supernatants similar amounts of leucine are incorporated into proteins of normal and dystrophic hamsters. However, using a dual labeling technique, a protein fraction is detected which shows different levels of leucine incorporation between normal and dystrophic animals in an age-dependent fashion. The major protein in this fraction comigrates with hemoglobin or its subunits under various conditions of polyacrylamide gel electrophoresis. In animals younger than 3 days there is more synthesis of this protein fraction in dystrophic animals, while at 6 days there is considerably less synthesis of this protein. These changes are paralleled by differences in the number of erythroid foci in histological sections of the livers. There is no evidence for structurally altered hemoglobin in dystrophic hamsters. The significance of this finding and its possible relation to the dystrophy process are not known at present.
在肝脏上清液中，正常仓鼠和营养不良仓鼠的蛋白质中掺入的亮氨酸量相似。然而，使用双重标记技术检测到一种蛋白质组分，该组分在正常动物和营养不良动物之间呈现出随年龄变化的不同亮氨酸掺入水平。在聚丙烯酰胺凝胶电泳的各种条件下，该组分中的主要蛋白质与血红蛋白或其亚基迁移率相同。在3日龄以下的动物中，营养不良动物中该蛋白质组分的合成较多，而在6日龄时，该蛋白质的合成则明显减少。肝脏组织切片中红细胞灶数量的差异与这些变化平行。没有证据表明营养不良仓鼠的血红蛋白结构发生改变。目前尚不清楚这一发现的意义及其与营养不良过程的可能关系。
