Cherry J M, Mackellar W, Morré D J, Crane F L, Jacobsen L B, Schirrmacher V
Biochim Biophys Acta. 1981 Jan 14;634(1):11-8. doi: 10.1016/0005-2728(81)90123-7.
A NADH-ferricyanide reductase of the external surface of intact mouse ascites tumor cells grown in culture was shown. The oxidation/reduction reaction was due to enzymatic rather than inorganic iron catalysis as demonstrated by the kinetics and specificity of the reaction. Activities of three markers for cytoplasmic contents were lacking with the intact tumor cells. The dehydrogenase activity was inhibited by p-chloromercuribenzoate, bathophenanthroline sulfonate, and the anticancer drug adriamycin. Sodium azide and potassium cyanide inhibited partially. The response to inhibitors resembled that of isolated plasma membranes rather than that of mitochondria. Concurrent with these findings, neither superoxide dismutase nor rotenone affected the redox activity. The findings provide evidence for the operation of a plasma membrane redox system at the surface of intact, living cells.
已证明在培养中生长的完整小鼠腹水肿瘤细胞外表面存在一种NADH-铁氰化物还原酶。氧化/还原反应是由酶催化而非无机铁催化引起的，这一点通过反应的动力学和特异性得到了证明。完整肿瘤细胞缺乏三种细胞质内容物标志物的活性。脱氢酶活性受到对氯汞苯甲酸、bathophenanthroline磺酸盐和抗癌药物阿霉素的抑制。叠氮化钠和氰化钾部分抑制。对抑制剂的反应类似于分离的质膜而非线粒体的反应。与这些发现同时，超氧化物歧化酶和鱼藤酮均不影响氧化还原活性。这些发现为完整活细胞表面质膜氧化还原系统的运作提供了证据。
