地西泮增强腺苷对离体心脏和平滑肌的作用。

Potentiation of the effects of adenosine on isolated cardiac and smooth muscle by diazepam.

Clanachan A S, Marshall R J

Br J Pharmacol. 1980;71(2):459-66. doi: 10.1111/j.1476-5381.1980.tb10958.x.

Abstract

Adenosine (10(-7) to 3 x 10(-4) M) or 2-chloroadenosine (10(-8) to 10(-5) M) produced concentration-dependent inhibition of the responses of the rat isolated vas deferens to electrical field stimulation. In electrically driven (2 Hz) guinea-pig isolated left atria, adenosine (10(-6) to 10(-3) M) or 2-chloroadenosine (10(-8) to 10(-7) M) produced concentration-dependent decreases in isometric tension. 2. Diazepam (10(-6) and 10(-5) M) had no direct effect per se, but significantly potentiated the inhibitory action of adenosine on both tissues without altering the inhibitory effect of 2-chloroadenosine. 3. The adenosine uptake inhibitors, hydroxynitrobenzylthioguanosine (HNBTG, 10(-5) M) and dipyridamole (10(-5) M) also potentiated the inhibitory actions of adenosine in rat vas deferens, but not hose of 2-chloroadenosine. 4. Following adenosine uptake inhibition in rat vas deferens by HNBTG (10(-5) M), diazepam (10(-5) M) failed to produce any significant further potentiation of the inhibitory action of adenosine. 5. It is concluded that the potentiation of adenosine by diazepam is possibly due to an inhibition of adenosine uptake.

摘要

腺苷（10⁻⁷至3×10⁻⁴M）或2 - 氯腺苷（10⁻⁸至10⁻⁵M）对大鼠离体输精管对电场刺激的反应产生浓度依赖性抑制。在电驱动（2Hz）的豚鼠离体左心房中，腺苷（10⁻⁶至10⁻³M）或2 - 氯腺苷（10⁻⁸至10⁻⁷M）使等长张力产生浓度依赖性降低。2. 地西泮（10⁻⁶和10⁻⁵M）本身无直接作用，但能显著增强腺苷对两种组织的抑制作用，而不改变2 - 氯腺苷的抑制作用。3. 腺苷摄取抑制剂羟基硝基苄基硫代鸟苷（HNBTG，10⁻⁵M）和双嘧达莫（10⁻⁵M）也增强了腺苷对大鼠输精管的抑制作用，但对2 - 氯腺苷的抑制作用无增强作用。4. 在大鼠输精管中，HNBTG（10⁻⁵M）抑制腺苷摄取后，地西泮（10⁻⁵M）未能进一步显著增强腺苷的抑制作用。5. 得出结论：地西泮对腺苷的增强作用可能是由于抑制了腺苷的摄取。