Mintz G, Gottlieb D I, Reitman M L, Derby M, Glaser L
Brain Res. 1981 Feb 9;206(1):51-70. doi: 10.1016/0006-8993(81)90100-1.
Temporal changes have been noted previously in retinal glycoproteins that bind to wheat germ agglutinin by a technique in which the denatured glycoproteins are first separated according to size by polyacrylamide gel electrophoresis, and are then localized on the gel using [125I]lectin. As reported here this technique will also detect differences between dorsal and ventral halves of the neural retina from 8-day chick embryos, and using other lectins will detect temporal changes in the glycoprotein pattern of the optic tectum. Some of the glycoproteins detected by wheat germ agglutinin in the neural retina appear to be represented on the surface of the retinal cells since: (a) the temporal changes in retinal glycoproteins can also be observed in a plasma membrane enriched fraction prepared from neural retina cells; and (b) antibodies prepared in mice against various size categories of wheat germ lectin binding glycoproteins bind to intact retinal cells.
以前曾通过一种技术注意到视网膜糖蛋白的时间变化，该技术是先通过聚丙烯酰胺凝胶电泳根据大小分离变性糖蛋白，然后使用[125I]凝集素将其定位在凝胶上。如本文所报道，该技术还将检测8日龄鸡胚神经视网膜背侧和腹侧两半之间的差异，并且使用其他凝集素将检测视顶盖糖蛋白模式的时间变化。小麦胚凝集素在神经视网膜中检测到的一些糖蛋白似乎存在于视网膜细胞表面，因为：(a)视网膜糖蛋白的时间变化也可以在从神经视网膜细胞制备的富含质膜的部分中观察到；(b)在小鼠中制备的针对各种大小类别的小麦胚凝集素结合糖蛋白的抗体与完整的视网膜细胞结合。
