Mintz G, Glaser L
J Cell Biol. 1978 Oct;79(1):132-7. doi: 10.1083/jcb.79.1.132.
After separation of whole proteins of chick neural retina by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate (SDS), a number of glycoproteins can be detected by staining the gels with 125I-labeled wheat germ agglutinin (WGA) and other lectins. The glycoprotein patterns show both quantitative and qualitative changes between days 7 and 13 of development. Some of these glycoproteins can be separated by chromatography on columns of insolubilized lectins. These observations suggest that purification of some of these glycoproteins identified by staining with radioactive lectins would yield retinal antigens which may be specific for developmental stage and cell type.
在十二烷基硫酸钠（SDS）存在的情况下，通过聚丙烯酰胺凝胶电泳分离鸡神经视网膜的全蛋白后，用125I标记的麦胚凝集素（WGA）和其他凝集素对凝胶进行染色，可检测到许多糖蛋白。糖蛋白模式在发育的第7天到第13天之间显示出定量和定性的变化。其中一些糖蛋白可以通过固定化凝集素柱色谱进行分离。这些观察结果表明，通过用放射性凝集素染色鉴定的一些糖蛋白的纯化将产生可能对发育阶段和细胞类型具有特异性的视网膜抗原。
