Wang N, Trend B, Bronson D L, Fraley E E
Cancer Res. 1980 Mar;40(3):796-802.
Trypsin G banding was performed on metaphase chromosomes from 14 cell lines derived from primary tumors or metastases of 11 patients with testicular cancer. Most of the cell lines, 11 of 14, have a modal number between 51 and 61. All lines have numerical and structural changes involving chromosome 1 with trisomy of the q arm being the common aberration. Break points in chromosome 1 were nonrandom, being concentrated in the regions of p12, q12, p36, and p22, which resulted in morphologically identical marker chromosomes in different cases. These changes probably are not artifacts of cell culture. In one instance, three lines derived from the same patient, one from tissue removed at operation, and two from separate metastases removed at autopsy nearly 3 years later after unsuccessful radiotherapy and chemotherapy had identical chromosome compositions. In another case, lines derived from a primary tumor and a metastasis from the same patient also had identical marker chromosomes. The consistent involvement of chromosome 1 in aberrations may be associated with the highly malignant nature of testicular cancers.
对来自11例睾丸癌患者原发性肿瘤或转移灶的14个细胞系的中期染色体进行了胰蛋白酶G显带分析。大多数细胞系（14个中的11个）的众数染色体数在51到61之间。所有细胞系均有涉及1号染色体的数目和结构改变，其中q臂三体是常见的畸变。1号染色体的断点是非随机的，集中在p12、q12、p36和p22区域，这导致不同病例中出现形态相同的标记染色体。这些改变可能不是细胞培养的假象。在一个实例中，来自同一患者的三个细胞系，一个来自手术切除的组织，另外两个来自近3年后尸检时分别切除的转移灶，当时放疗和化疗均未成功，它们具有相同的染色体组成。在另一个病例中，来自同一患者原发性肿瘤和转移灶的细胞系也有相同的标记染色体。1号染色体持续参与畸变可能与睾丸癌的高度恶性性质有关。
