Martinez J C, Mateo M, Sánchez-Beato M, Villuendas R, Orradre J L, Algara P, Sánchez-Verde L, García P, López C, Martínez P
Department of Pathology, Hospital Virgen de la Salud, Toledo, Spain.
J Pathol. 1995 Sep;177(1):27-34. doi: 10.1002/path.1711770106.
MDM2 and p53 immunohistochemical protein expression was analysed in lymphocytes and in reactive and neoplastic lymphoid tissue. Phytohaemagglutinin (PHA)-stimulated lymphocytes displayed MDM2 and p53 co-expression. In 8 of 8 tonsils, 24 of 24 Hodgkin's disease (HD), and 10 of 24 high-grade non-Hodgkin's lymphoma (HG-NHL) specimens, MDM2 paralleled p53 nuclear expression in non-tumour and tumour cells. The number of positive cells was greater and the staining intensity was stronger for p53 than for MDM2. In another nine of the 24 HG-NHL cases studied, dissociated expression was observed, with high p53 expression and very low or absent MDM2 expression. In five cases, both MDM2 and p53 were negative. The eight low-grade NHL (LG-NHL) cases were also MDM2- and p53-negative. MDM2 and p53 expression in PHA-activated lymphocytes and reactive lymphoid tissue is probably an expression of opposing biological signals regulating cell proliferation. Parallel MDM2 and p53 expression in all HD and in 10 out of 24 HG/NHL cases may indicate that this growth suppressive pathway is maintained in those cases. However, dissociated MDM2/p53 expression (nine cases) and the absence of expression of both proteins (five cases) may represent examples of deregulation of this growth control pathway. These findings are in agreement with previous in vitro studies in cell lines regarding the role of MDM2/p53 lymphoid tissue, suggesting a possible role for MDM2 deregulation in lymphomagenesis.
在淋巴细胞以及反应性和肿瘤性淋巴组织中分析了MDM2和p53免疫组化蛋白表达。植物血凝素（PHA）刺激的淋巴细胞显示出MDM2和p53的共表达。在8个扁桃体、24个霍奇金淋巴瘤（HD）标本中的24个以及24个高级别非霍奇金淋巴瘤（HG-NHL）标本中的10个中，MDM2与非肿瘤细胞和肿瘤细胞中的p53核表达平行。p53阳性细胞数量更多，染色强度比MDM2更强。在研究的24例HG-NHL病例中的另外9例中，观察到表达解离，p53高表达而MDM2表达极低或无表达。在5例中，MDM2和p53均为阴性。8例低级别NHL（LG-NHL）病例MDM2和p53也均为阴性。PHA激活的淋巴细胞和反应性淋巴组织中MDM2和p53的表达可能是调节细胞增殖的相反生物信号的一种表达。所有HD以及24例HG/NHL病例中的10例中MDM2和p53的平行表达可能表明在这些病例中这种生长抑制途径得以维持。然而，MDM2/p53表达解离（9例）以及两种蛋白均无表达（5例）可能代表了这种生长控制途径失调的例子。这些发现与先前关于MDM2/p53在淋巴组织中作用的细胞系体外研究结果一致，提示MDM2失调在淋巴瘤发生中可能起作用。
