Jahnukainen K, Attermo P, Saari T, Salmi T T, von Euler M, Pöllänen P, Söder O
Department of Anatomy, University of Turku, Finland.
J Reprod Immunol. 1995 Mar;28(3):263-73. doi: 10.1016/0165-0378(95)00926-c.
A rat acute lymphoblastic leukaemia (ALL) model was used to study the mechanisms involved in the tendency to testicular relapse of ALL in boys. Previous studies have indicated that the infiltration and growth of leukaemic lymphoblasts in the testis are influenced by the same endocrine and paracrine control systems that regulate normal testicular function. In the present study the effects of aqueous extracts of scrotal, abdominal and estrogen-treated postpubertal rat testes on rat-leukaemic lymphoblast proliferation were evaluated. The effects of recombinant cytokines analogous to those observed in the testis on leukaemic cell DNA-synthesis were also evaluated since changes in the levels of these factors have been observed in association with cryptorchidism and low levels of gonadotropins. Transforming growth factor-beta 1 (TGF-beta1), significantly inhibited the proliferation of leukaemic rat lymphoblasts after 24 h of culture, whereas TGF-beta 2, interleukin-1 alpha (IL-1 alpha), IL-1 beta, IL-6 or combinations of them were inactive. Extracts of estrogen-treated testes and abdominal testes of unilaterally cryptorchid animals inhibited leukaemic T-cell proliferation significantly more than extracts of normal testes. The inhibitory activity in abdominal testes could be neutralized by anti-TGF-beta 1 antibodies. These results suggest that testicular TGF-beta 1 may influence growth of leukaemic lymphoblasts in the testis but also that other as yet unknown, testicular factors are involved in the regulation of leukaemic cell function in the testis.
利用大鼠急性淋巴细胞白血病（ALL）模型研究男孩ALL睾丸复发倾向的相关机制。以往研究表明，白血病原始淋巴细胞在睾丸中的浸润和生长受调控正常睾丸功能的相同内分泌和旁分泌控制系统影响。在本研究中，评估了青春期后大鼠阴囊、腹部及经雌激素处理的睾丸水提取物对大鼠白血病原始淋巴细胞增殖的影响。还评估了与睾丸中观察到的类似的重组细胞因子对白血病细胞DNA合成的影响，因为在隐睾症和促性腺激素水平低的情况下已观察到这些因子水平的变化。培养24小时后，转化生长因子-β1（TGF-β1）显著抑制白血病大鼠原始淋巴细胞的增殖，而TGF-β2、白细胞介素-1α（IL-1α）、IL-1β、IL-6或它们的组合则无活性。单侧隐睾动物经雌激素处理的睾丸和腹部睾丸的提取物对白血病T细胞增殖的抑制作用明显强于正常睾丸提取物。腹部睾丸中的抑制活性可被抗TGF-β1抗体中和。这些结果表明，睾丸TGF-β1可能影响白血病原始淋巴细胞在睾丸中的生长，但也表明其他尚未明确的睾丸因子参与睾丸中白血病细胞功能的调节。
