Anderson R A, Brust R A
Department of Entomology, University of Manitoba, Winnipeg, Canada.
J Med Entomol. 1995 Sep;32(5):705-10. doi: 10.1093/jmedent/32.5.705.
Field studies were conducted in Manitoba, Canada, and Florida, United States to evaluate the frequency of multiple host contacts by Culex tarsalis Coquillett, Cx. restuans Theobald, and Cx. nigripalpus Theobald, primary and secondary vectors of several encephalitis viruses in North America. Blood-fed mosquitoes were collected from box traps, each baited with a pair of quail. One quail of each pair was injected with rubidium and the other with cesium to permit the determination of the source(s) of each blood meal. Approximately 5% of all blood-fed Cx. tarsalis, Cx. restuans, and Cx. nigripalpus ingested blood from both quail during overnight exposure. The frequency of multiple feeding by these species ranged from 0 to 18.5%, 0 to 33.3%, and 0 to 17.6%, respectively. Of the 331 mosquitoes that ingested blood from 2 quail, 57 (17.2%) were not fully blood fed. Also, 1.0-3.5% of blood-fed mosquitoes had ingested blood before entering the quail-baited traps. Any increase in the number of host contacts as a result of multiple feeding, however modest, may increase disproportionately the rate at which virus transmission occurs.
在加拿大曼尼托巴省和美国佛罗里达州开展了实地研究，以评估北美几种脑炎病毒的主要和次要传播媒介——环跗库蚊、雷氏库蚊和黑跗库蚊与多个宿主接触的频率。从箱式诱捕器中收集吸食了血液的蚊子，每个诱捕器都用一对鹌鹑作为诱饵。每对鹌鹑中的一只注射铷，另一只注射铯，以便确定每顿血餐的来源。在夜间暴露期间，所有吸食了血液的环跗库蚊、雷氏库蚊和黑跗库蚊中，约5%从两只鹌鹑身上都吸食了血液。这些种类的多次吸食频率分别为0%至18.5%、0%至33.3%和0%至17.6%。在从两只鹌鹑身上吸食了血液的331只蚊子中，有57只（17.2%）没有完全饱血。此外，1.0%至3.5%吸食了血液的蚊子在进入用鹌鹑作诱饵的诱捕器之前就已经吸食过血液。然而，由于多次吸食导致的宿主接触次数的任何增加，无论多么微小，都可能不成比例地增加病毒传播的速度。
