Bernier R, Turco S J, Olivier M, Tremblay M
Centre de Recherche en Infectiologie, Centre Hospitalier de l'Université Laval, Faculté de Médecine, Université Laval, Ste-Foy Québec, Canada.
J Virol. 1995 Nov;69(11):7282-5. doi: 10.1128/JVI.69.11.7282-7285.1995.
In this study, we demonstrated that the protozoan parasite Leishmania donovani and one of its major surface molecules, the lipophosphoglycan (LPG), can induce human immunodeficiency virus type 1 (HIV-1) expression in U1 and OM-10.1, two cell lines of monocytoid origin latently infected with HIV-1. Treatment of U1 cells with various concentrations of LPG (1, 5, and 10 microM) resulted in a dose-dependent secretion of tumor necrosis factor alpha (TNF-alpha). Suppression of LPG-induced HIV-1 expression by polyclonal anti-TNF-alpha antibodies further confirmed the involvement of this cytokine. Results from these studies indicate that the protozoan parasite L. donovani can induce the secretion of TNF-alpha that will function in an autocrine or paracrine manner to upregulate HIV-1 expression. Our data suggest for the first time that this protozoan parasite can be viewed as a potential cofactor in the pathogenesis of AIDS.
在本研究中，我们证明原生动物寄生虫杜氏利什曼原虫及其主要表面分子之一脂磷壁酸聚糖（LPG），可在U1和OM-10.1这两种单核细胞系来源且潜伏感染了1型人类免疫缺陷病毒（HIV-1）的细胞系中诱导HIV-1表达。用不同浓度的LPG（1、5和10微摩尔）处理U1细胞，会导致肿瘤坏死因子α（TNF-α）呈剂量依赖性分泌。多克隆抗TNF-α抗体对LPG诱导的HIV-1表达的抑制作用进一步证实了这种细胞因子的参与。这些研究结果表明，原生动物寄生虫杜氏利什曼原虫可诱导TNF-α的分泌，TNF-α将以自分泌或旁分泌方式发挥作用，上调HIV-1表达。我们的数据首次表明，这种原生动物寄生虫可被视为艾滋病发病机制中的一种潜在辅助因子。
