Maeda M
School of Pharmaceutical Sciences, Showa University.
Nihon Rinsho. 1995 Sep;53(9):2140-2.
The preparation of solid phase immobilized antibody or antigen was physical adsorption or chemical binding using coupling reagent such as glutalaldehyde etc. The concentration of antibody or antigen on solid phase was effective to sensitivity of enzyme immunoassay. When the nonspecific binding of enzyme conjugate to solid phase was minimized, the sensitivity of enzyme immunoassay will be succeed in higher.
固相固定化抗体或抗原的制备是通过物理吸附或使用诸如戊二醛等偶联试剂进行化学结合。固相上抗体或抗原的浓度对酶免疫测定的灵敏度有影响。当酶结合物与固相的非特异性结合降至最低时，酶免疫测定的灵敏度将成功提高。
