Uchimura H
Central Laboratory, Tokyo University Hospital.
Nihon Rinsho. 1995 Sep;53(9):2245-9.
Recently various non-radioisotopic immunoassays have been developed instead of radioisotopic assay and widely used in the laboratory. Enzyme immunoassay (EIA) is most popular in use including chemiluminescent enzyme immunoassay (CLEIA). At present few hormones (thyroid related and gonadotropic hormones) are measured by EIA with semi or full automatic analysers and satisfactory specificity and sensitivity were already obtained. However, most hormones are still measured by RIA or IRMA. EIA for these hormones should be developed. Standardization of reference interval and calibrators would also be needed for the compatibility between different assays.
近年来，各种非放射性免疫分析方法已被开发出来取代放射性分析方法，并在实验室中广泛使用。酶免疫分析（EIA）应用最为广泛，包括化学发光酶免疫分析（CLEIA）。目前，少数激素（甲状腺相关激素和促性腺激素）通过半自动或全自动分析仪进行酶免疫分析测定，已经获得了令人满意的特异性和灵敏度。然而，大多数激素仍通过放射免疫分析（RIA）或免疫放射分析（IRMA）进行测定。因此，需要开发针对这些激素的酶免疫分析方法。为了使不同分析方法具有兼容性，还需要对参考区间和校准物进行标准化。
