Kikuchi T, Takeda Y, Shimizu A
Central Clinical Laboratory, Osaka Medical College Hospital, Takatsuki.
Rinsho Byori. 1995 Sep;43(9):917-24.
Accompanying hematology laboratory automation, the frequency of morphological observation of blood cells tends to decrease. Although 5-part differentials are performed by automated systems, counting of abnormal cells such as immature cells or leukemia cells still must be performed manually using smears. In the automated hematology laboratories, technicians more frequently find abnormal cells than before. Therefore, poor microscopic observation of blood cells markedly affects the diagnosis. Continuous training of the technologist is mandatory. Also, preparation of smear samples is important, i.e, well prepared slides must be stained properly with an appropriate dye. Technicians must be familiar with staining technology together with the knowledge of the characteristics of several staining methods. On the other hand, the technician's ability to differentiate white blood cells depends on the instructor's knowledge. This makes quality control and the standardization of differentials difficult. Still the problems must be solved under the cooperation of the various societies concerned.
随着血液学实验室自动化的发展，血细胞形态学观察的频率趋于降低。尽管自动系统可进行五分类计数，但对未成熟细胞或白血病细胞等异常细胞的计数仍需使用涂片进行手工操作。在自动化血液学实验室中，技术人员比以往更频繁地发现异常细胞。因此，血细胞显微镜观察不佳会显著影响诊断。技术人员必须持续接受培训。此外，涂片样本的制备也很重要，即制备良好的玻片必须用适当的染料进行正确染色。技术人员必须熟悉染色技术以及几种染色方法的特点。另一方面，技术人员区分白细胞的能力取决于指导教师的知识。这使得分类计数的质量控制和标准化变得困难。尽管如此，相关各方仍需合作解决这些问题。
