Wree A, Goller H J, Beck T
Institut für Anatomie, Universität Rostock, Germany.
J Neurosci Methods. 1995 May;58(1-2):143-9. doi: 10.1016/0165-0270(94)00168-g.
Local cerebral glucose utilization (LCGU) was measured in 75 cortical areas and nuclei of adult, 3-4-month-old Wistar rats, using the [14C]2-deoxyglucose (2-DG) technique. Measurement of total brain radioactivity content was not significantly different in unfixed material compared to fixed brain tissue. Values of LCGU derived from fresh, unfixed material were compared with values obtained from rats fixed by perfusion 45 min after the [14C]2-DG bolus injection with phosphate-buffered 3.3% paraformaldehyde at room temperature. In the fixed material, the mean LCGU of all brain regions was significantly increased by about 25% compared with the unfixed specimen due to tissue shrinkage of 7.2% in the fixed brains. Shrinkage leading to a higher volume density of [14C]2-deoxyglucose-6-phosphate in brain tissue results in a higher grain density in the respective autoradiographs. The wash-out of blood-borne [14C]2-DG is negligible except for blood-rich structures like the pineal gland and the choroid plexus.
采用[14C]2-脱氧葡萄糖（2-DG）技术，在3至4月龄成年Wistar大鼠的75个皮质区域和核团中测量局部脑葡萄糖利用（LCGU）。与固定脑组织相比，未固定材料中全脑放射性含量的测量无显著差异。将新鲜未固定材料获得的LCGU值与在[14C]2-DG团注注射后45分钟，于室温下用磷酸盐缓冲的3.3%多聚甲醛灌注固定的大鼠所获得的值进行比较。在固定材料中，由于固定脑的组织收缩7.2%，所有脑区的平均LCGU与未固定标本相比显著增加约25%。收缩导致脑组织中[14C]2-脱氧葡萄糖-6-磷酸的体积密度更高，从而在各自的放射自显影片中产生更高的颗粒密度。除了像松果体和脉络丛这样富含血液的结构外，血源性[14C]2-DG的洗脱可以忽略不计。
