Zhuang S M, Landegent J E, Verschueren C A, Falkenburg J H, van Ormondt H, van der Eb A J, Noteborn M H
Laboratory of Molecular Carcinogenesis, Sylvius Laboratory, Leiden University, The Netherlands.
Leukemia. 1995 Oct;9 Suppl 1:S118-20.
Apoptin, a small protein encoded by chicken anemia virus (CAV) was expressed in various human hematologic malignant cell lines derived from leukemias and lymphoma. Three of these cell lines contain bcl-2 or BCR-ABL proteins, known to block apoptosis induced by chemotherapeutic compounds. By immunofluorescence and propidium-iodide staining apoptin was shown to induce apoptosis in all analysed cell lines. Early after expression, apoptin exhibited a fine-granular distribution in the still intact nucleus. Later, apoptin became aggregated and the nucleus segmented. The data with truncated apoptin indicate that for optimal induction of apoptosis apoptin has to be located in the nucleus.
凋亡素是一种由鸡贫血病毒（CAV）编码的小蛋白，它在源自白血病和淋巴瘤的多种人类血液系统恶性细胞系中表达。其中三个细胞系含有已知可阻断化疗化合物诱导的细胞凋亡的bcl-2或BCR-ABL蛋白。通过免疫荧光和碘化丙啶染色显示，凋亡素可在所有分析的细胞系中诱导细胞凋亡。在表达后早期，凋亡素在仍然完整的细胞核中呈现细颗粒状分布。后来，凋亡素聚集，细胞核发生分割。截短的凋亡素的数据表明，为了最佳地诱导细胞凋亡，凋亡素必须位于细胞核中。
