哺乳动物尿素合成中氨的来源。
Sources of ammonia for mammalian urea synthesis.
作者信息
Krebs H A, Hems R, Lund P, Halliday D, Read W W
出版信息
Biochem J. 1978 Dec 15;176(3):733-7. doi: 10.1042/bj1760733.
Abstract
The initial rate of incorporation of [15N]alanine into the 6-amino group of the adenine nucleotides in rat hepatocytes was about one-eighteenth of the rate of incorporation into urea. Thus the purine nucleotide cycle cannot provide most of the ammonia needed in urea synthesis for the carbamoyl phosphate synthase reaction (EC 2.7.2.5). On the other hand, contrary to the view expressed by McGivan & Chappell [(1975) FEBS Lett. 52, 1--7], the experiments support the view that hepatic glutamate dehydrogenase can supply the required ammonia.
摘要
[15N]丙氨酸掺入大鼠肝细胞腺嘌呤核苷酸6-氨基的初始速率约为掺入尿素速率的十八分之一。因此，嘌呤核苷酸循环无法为氨甲酰磷酸合酶反应（EC 2.7.2.5）提供尿素合成所需的大部分氨。另一方面，与McGivan和Chappell [(1975) FEBS Lett. 52, 1 - 7]所表达的观点相反，这些实验支持肝脏谷氨酸脱氢酶能够提供所需氨的观点。
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