L-亮氨酸对离体大鼠肝细胞尿素、谷氨酸和谷氨酰胺合成的影响。
The effects L-leucine on the synthesis of urea, glutamate and glutamine by isolated rat liver cells.
作者信息
Mourão J M, McGivan J D, Chappell J B
出版信息
Biochem J. 1975 Feb;146(2):457-64. doi: 10.1042/bj1460457.
Abstract
With either alanine or a mixture of 15 different amino acids as nitrogen source, the addition of L-leucine inhibited the synthesis of urea by isolated rat liver cells. With alanine present leucine promoted the production of glutamate and glutamine. Comparison of effects of leucine on soluble glutamate dehydrogenase, mitochondria and isolated cells supports the postulate that leucine exerts its effect through activation of glutamate dehydrogenase. It is suggested that this latter enzyme may not be as important for the production of NH3 for carbamoyl phosphate synthesis as has been considered hitherto.
摘要
以丙氨酸或15种不同氨基酸的混合物作为氮源时，添加L-亮氨酸会抑制离体大鼠肝细胞的尿素合成。当存在丙氨酸时，亮氨酸会促进谷氨酸和谷氨酰胺的生成。亮氨酸对可溶性谷氨酸脱氢酶、线粒体和离体细胞的作用比较支持了这样一种假设，即亮氨酸通过激活谷氨酸脱氢酶发挥其作用。有人提出，后一种酶对于为氨甲酰磷酸合成提供氨的作用可能不像迄今为止所认为的那么重要。
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