Ferré P, Pégorier J P, Williamson D H, Girard J R
Biochem J. 1978 Dec 15;176(3):759-65. doi: 10.1042/bj1760759.
In the suckling newborn rat, blood ketone bodies begin to increase slowly 4h after birth and then rise sharply between 12 and 16h, whereas the major increase in plasma non-esterified fatty acids and liver carnitine occurs during the first 2h of life, parallel with the onset of suckling. In the starved newborn rat, which shows no increase in liver carnitine unless it is fed with a carnitine solution, the developmental pattern of the ketogenic capacity (tested by feeding a triacylglycerol emulsion, which increases plasma non-esterified fatty acids by 3-fold) is the same as in the suckling animal. This suggests that the increases in plasma non-esterified fatty acids and liver carnitine seen 2h after birth in the suckling animal are not the predominant factors inducing the switch-on of ketogenesis. Injection of butyrate to starved newborn pups resulted in a pattern of blood ketone bodies which was similar to that found after administration of triacylglycerols, but, at all time points studied, the hyperketonaemia was more pronounced with butyrate. It is suggested that, even if the entry of long-chain fatty acids into the mitochondria is a rate-limiting step, it is not the only factor controlling ketogenesis after birth in the rat. As in the adult rat, there is a reciprocal correlation between the liver glycogen content and the concentration of ketone bodies in the blood.
在哺乳的新生大鼠中，出生后4小时血酮体开始缓慢增加，然后在12至16小时之间急剧上升，而血浆非酯化脂肪酸和肝脏肉碱的主要增加发生在出生后的前2小时，与哺乳开始同步。在饥饿的新生大鼠中，除非喂食肉碱溶液，肝脏肉碱不会增加，其生酮能力的发育模式（通过喂食三酰甘油乳剂进行测试，该乳剂可使血浆非酯化脂肪酸增加3倍）与哺乳动物相同。这表明，在哺乳动物出生后2小时观察到的血浆非酯化脂肪酸和肝脏肉碱的增加并非诱导生酮开启的主要因素。给饥饿的新生幼崽注射丁酸盐后，血酮体模式与注射三酰甘油后相似，但在所有研究时间点，丁酸盐导致的高酮血症更为明显。有人认为，即使长链脂肪酸进入线粒体是一个限速步骤，但它不是控制大鼠出生后生酮的唯一因素。与成年大鼠一样，肝脏糖原含量与血液中酮体浓度之间存在负相关。
