哺乳期兔乳腺中含葡萄糖的脂连接寡糖。
Lipid-linked oligosaccharides containing glucose in lactating rabbit mammary gland.
作者信息
Speake B K, White D A
出版信息
Biochem J. 1978 Dec 15;176(3):993-1000. doi: 10.1042/bj1760993.
Abstract
- Microsomal fractions of lactating rabbit mammary gland incubated with UDP-glucose formed lipid-linked mono- and oligo-saccharides. The lipid-linked monosaccharide had chromatographic properties similar to those of dolichol phosphate mannose and yielded glucose on acid hydrolysis. 2. Incubation of the microsomal fraction with GDP-[U14C]-mannose yielded an oligosaccharide lipid of approximately seven monosaccharide units. Further incubation with UDP-glucose increased the size of the oligosaccharide by approximately two units. 3. Explants of lactating rabbit mammary gland incorporated [U-14C]glucose into both lipid-linked mono- and oligo-saccharides. The oligosaccharide lipid was of approx. 11 monosaccharide units. 4. Considerable redistribution of radioactive label occurred in the explant system, and radioactively labelled glucosamine and mannose, as well as glucose, were detected on acid hydrolysis of the oligosaccharide lipid. 5. Glucose was also detected in the acid hydrolysate of explant proteins. Radioactive glucosamine, galactosamine, galactose and mannose were also found in this fraction.
摘要
- 用UDP-葡萄糖孵育泌乳兔乳腺的微粒体部分，形成了脂质连接的单糖和寡糖。该脂质连接的单糖具有与磷酸多萜醇甘露糖相似的色谱性质，在酸性水解时产生葡萄糖。2. 用GDP-[U14C]-甘露糖孵育微粒体部分，产生了一种约含七个单糖单位的寡糖脂质。进一步用UDP-葡萄糖孵育，使寡糖大小增加约两个单位。3. 泌乳兔乳腺外植体将[U-14C]葡萄糖掺入脂质连接的单糖和寡糖中。该寡糖脂质约含11个单糖单位。4. 在该外植体系统中发生了放射性标记的大量重新分布，在寡糖脂质的酸性水解产物中检测到放射性标记的葡糖胺、甘露糖以及葡萄糖。5. 在乳腺外植体蛋白的酸性水解产物中也检测到了葡萄糖。在该部分中还发现了放射性葡糖胺、半乳糖胺、半乳糖和甘露糖。
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