使用安德森采样器收集的空气中颗粒物中硝基芘的分布及致突变性

Distributions of nitropyrenes and mutagenicity in airborne particulates collected with an Andersen sampler.

作者信息

Hayakawa K, Kawaguchi Y, Murahashi T, Miyazaki M

机构信息

Faculty of Pharmaceutical Sciences, Kanazawa University, Japan.

出版信息

Mutat Res. 1995 Oct;348(2):57-61. doi: 10.1016/0165-7992(95)00046-1.

DOI:10.1016/0165-7992(95)00046-1

PMID:7477052

Abstract

Distributions of 1,3-dinitropyrene (1,3-DNP), 1,6-DNP, 1,8-DNP, 1-nitropyrene (1-NP) and mutagenicity in airborne particulates collected in downtown Kanazawa, Japan with an Andersen high-volume air sampler were examined. Mutagenicities of benzene-ethanol extract from particulates were determined by the Ames test using S. typhimurium strains without S9 mix, while concentrations of DNPs and 1-NP were determined by high-performance liquid chromatography (HPLC) using chemiluminescence detection. In the finest particulate fraction (smaller than 1.1 microns), 68% and 75% of the total mutagenicities were observed in TA98 and YG1024 strains, respectively. In the same fraction, 65-82% of three DNPs as well as 84% of 1-NP were observed. Mutagenic contributions of 1,3-DNP, 1,6-DNP, 1,8-DNP and 1-NP in the extract were respectively 0.6, 1.2, 1.8 and 1.6% in the TA98 strain, and 2.5, 5, 9 and 2.1% in the YG1024 strain.

摘要

使用安德里森大容量空气采样器，对在日本金泽市中心采集的空气颗粒物中1,3 - 二硝基芘（1,3 - DNP）、1,6 - DNP、1,8 - DNP、1 - 硝基芘（1 - NP）的分布及致突变性进行了研究。颗粒物苯 - 乙醇提取物的致突变性通过使用无S9混合液的鼠伤寒沙门氏菌菌株的艾姆斯试验来测定，而DNPs和1 - NP的浓度则通过使用化学发光检测的高效液相色谱法（HPLC）来测定。在最细的颗粒物组分（小于1.1微米）中，TA98和YG1024菌株分别观察到总致突变性的68%和75%。在同一组分中，观察到三种DNPs的65 - 82%以及1 - NP的84%。提取物中1,3 - DNP、1,6 - DNP、1,8 - DNP和1 - NP在TA98菌株中的致突变贡献分别为0.6%、1.2%、1.8%和1.6%，在YG1024菌株中分别为2.5%、5%、9%和2.1%。