Cheng T J, Christiani D C, Wiencke J K, Wain J C, Xu X, Kelsey K T
Department of Cancer Biology, Harvard School of Public Health, Boston, MA 02115, USA.
Mutat Res. 1995 Oct;348(2):75-82. doi: 10.1016/0165-7992(95)00049-6.
Cytogenetic alterations have been associated with the occurrence of many cancers. However, limited data exist to address whether increased chromosomal changes in surrogate normal tissue are similarly associated with malignancy. As part of an ongoing case-control study of lung cancer, we have studied the factors that affect sister chromatid exchange (SCE) frequency in lymphocytes from lung cancer patients. Further, we sought to investigate whether the factors that affect SCE frequencies were comparable in lung cancer cases and controls. Cases had newly diagnosed, operable primary lung cancer. Controls were friends and spouses of cases. Detailed information on smoking, family history of cancer, medical history, and environmental and occupational exposures was obtained in an interviewer-administered questionnaire. Intake of antioxidants was also determined through the administration of a validated semiquantitative food frequency questionnaire. Metabolic traits studied included the polymorphic glutathione-S-transferase class mu (GST-mu) and variants of P450 isoenzymes CYP1A1 and CYP 2D6. Overall, 78 cases and 78 controls were included in the analysis. Although there was a small number of lung cancer patients who had never smoked in the study (9% of cases), these patients had higher SCE frequencies than current or former smokers. This suggests that factors associated with genomic instability may also play a role in the pathogenesis of lung cancer. The best fit model for SCE frequency, which had been previously generated from control data alone, included age, gender, smoking, GST-mu, and vitamin A intake. However, when this model was applied to lung cancer patients, smoking was not associated with an elevated SCE frequency. Thus, it is not clear that SCE frequency data in prevalent lung cancer cases and controls are comparable.
细胞遗传学改变与许多癌症的发生有关。然而，关于替代正常组织中染色体变化增加是否同样与恶性肿瘤相关的数据有限。作为一项正在进行的肺癌病例对照研究的一部分，我们研究了影响肺癌患者淋巴细胞中姐妹染色单体交换（SCE）频率的因素。此外，我们试图调查影响SCE频率的因素在肺癌病例和对照中是否具有可比性。病例为新诊断的、可手术的原发性肺癌患者。对照为病例的朋友和配偶。通过访谈员管理的问卷获取了关于吸烟、癌症家族史、病史以及环境和职业暴露的详细信息。抗氧化剂的摄入量也通过一份经过验证的半定量食物频率问卷来确定。所研究的代谢特征包括多态性谷胱甘肽 - S转移酶μ类（GST - μ）以及细胞色素P450同工酶CYP1A1和CYP 2D6的变体。总体而言，78例病例和78例对照纳入了分析。尽管在该研究中有少量从未吸烟的肺癌患者（占病例的9%），但这些患者的SCE频率高于当前或既往吸烟者。这表明与基因组不稳定相关的因素可能也在肺癌的发病机制中起作用。之前仅根据对照数据生成的SCE频率最佳拟合模型包括年龄、性别、吸烟、GST - μ和维生素A摄入量。然而，当将该模型应用于肺癌患者时，吸烟与升高的SCE频率无关。因此，尚不清楚现患肺癌病例和对照中的SCE频率数据是否具有可比性。
