Nelson L M, Parkinson D
Can J Microbiol. 1978 Dec;24(12):1468-74. doi: 10.1139/m78-236.
Three isolates, a Pseudomonas sp., Bacillus sp., and Arthrobacter sp., which had been isolated from a meadow soil at Devon Island, Canada, were subjected to freezing and thawing at low rates under various conditions. When cells were frozen in sand or soil, survival was dependent on moisture level, storage time, and thaw rate. Pseudomonas M216 was most susceptible to freeze-thaw damage under these conditions. Arthrobacter M51 was the most resistant of the three isolates when frozen in sand or soil and when frozen at a high rate after growth at varing rates at 5 and 15 degrees C in carbon-or nitrogen-limited media. Pseudomonas M216 was more sensitive to freeze-thaw damage when NaCl was present in the freezing menstruum, even at low freezing rates. Survival of cells frozen in growth medium, water, saline, and soil extract was not affected by the freezing rate when it was less than 1 degrees C min-1. Soil extract did not protect cells from freeze-thaw damage any more than water and for Arthrobacter M51 survival was decreased when it was frozen in soil extract.
从加拿大德文岛的一片草地土壤中分离出的三种菌株，即假单胞菌属、芽孢杆菌属和节杆菌属，在各种条件下以较低速率进行冷冻和解冻处理。当细胞在沙子或土壤中冷冻时，存活率取决于水分含量、储存时间和解冻速率。在这些条件下，假单胞菌M216对冻融损伤最为敏感。当在沙子或土壤中冷冻以及在5℃和15℃下于碳或氮限制培养基中以不同速率生长后高速冷冻时，节杆菌M51是这三种分离菌株中最具抗性的。当冷冻介质中存在氯化钠时，即使在低冷冻速率下，假单胞菌M216对冻融损伤也更敏感。当冷冻速率小于1℃/分钟时，在生长培养基、水、盐水和土壤提取物中冷冻的细胞存活率不受冷冻速率的影响。土壤提取物对细胞免受冻融损伤的保护作用并不比水更好，而且对于节杆菌M51来说，当它在土壤提取物中冷冻时存活率会降低。
