Kagan J, Stein J, Babaian R J, Joe Y S, Pisters L L, Glassman A B, von Eschenbach A C, Troncoso P
Division of Laboratory Medicine-Hematopathology Program, University of Texas, M.D. Anderson Cancer Center, Houston 77030, USA.
Oncogene. 1995 Nov 16;11(10):2121-6.
Frequent loss of an allele at specific chromosomal regions implicates these regions as sites of tumor suppressor genes (TSG) that become inactivated during tumor progression. We have studied chromosome 8p allele losses in 32 primary human prostate carcinomas with 16 polymorphic microsatellite sequences. Overall, 22 of 32 (69%) informative specimens showed loss of allele in at least one locus. The most frequent losses of heterozygosity (LOH) occurred at the LPL locus (46%) on chromosome 8p22 and at the D8S360 (45%) and NEFL (43%) loci on chromosome 8p21. Homozygous deletions were detected at the LPL and NEFL loci at 8p22 and 8p21, respectively. The minimal region with frequent LOH and homozygous deletion, around the LPL locus, was restricted between the MSR locus and the D8S258 marker, separated by less than 9 cM. The second region was restricted between markers D8S1128 and D8S131 separated by 12 cM. The results suggest the existence of two chromosome 8p sites for candidate TSGs in prostate cancer.
特定染色体区域上等位基因的频繁缺失表明这些区域是肿瘤抑制基因（TSG）的所在位点，这些基因在肿瘤进展过程中会失活。我们使用16个多态性微卫星序列研究了32例原发性人类前列腺癌中的8号染色体p等位基因缺失情况。总体而言，32例信息充分的标本中有22例（69%）至少在一个位点显示等位基因缺失。杂合性缺失（LOH）最常见于8号染色体p22上的LPL位点（46%）以及8号染色体p21上的D8S360（45%）和NEFL（43%）位点。分别在8号染色体p22和p21的LPL和NEFL位点检测到纯合缺失。LPL位点周围频繁出现LOH和纯合缺失的最小区域局限于MSR位点和D8S258标记之间，间隔小于9厘摩。第二个区域局限于间隔12厘摩的D8S1128和D8S131标记之间。结果表明在前列腺癌中8号染色体p上存在两个候选TSG位点。
