Prasad M A, Trybus T M, Wojno K J, Macoska J A
Department of Surgery, Comprehensive Cancer Center, University of Michigan, Ann Arbor 48109-0946, USA.
Genes Chromosomes Cancer. 1998 Nov;23(3):255-62.
By using tissue microdissection and polymerase chain reaction (PCR) techniques, we examined 85 prostate tumors that were paired with normal tissues from the same patients for allelic loss at 26 highly polymorphic microsatellite sequences, 21 spanning 8p and 5 localized to 8q. Sixty-four tumors (75%) demonstrated loss of at least one 8p locus. Separate distal and proximal regions of deletion were observed as well as an intervening, staggered breakpoint. A novel region of homozygous deletion of sequences at the D8S87 locus was detected both by multiplex PCR and by fluorescence in situ hybridization within this breakpoint region. These data suggest that a tumor-suppressor gene mapping to proximal 8p is deleted frequently and is likely to be important for tumorigenesis in prostate tumors.
通过使用组织显微切割和聚合酶链反应（PCR）技术，我们检测了85例前列腺肿瘤，这些肿瘤与来自同一患者的正常组织配对，检测26个高度多态性微卫星序列的等位基因缺失情况，其中21个位于8p，5个位于8q。64例肿瘤（75%）显示至少一个8p位点缺失。观察到单独的远端和近端缺失区域以及一个中间的交错断点。通过多重PCR和荧光原位杂交在该断点区域内均检测到D8S87位点序列的一个新的纯合缺失区域。这些数据表明，定位于8p近端的一个肿瘤抑制基因经常缺失，可能对前列腺肿瘤的发生发展很重要。
