Pan C, Mason T L
Department of Biochemistry and Molecular Biology, University of Massachusetts, Amherst 01003, USA.
Nucleic Acids Res. 1995 Sep 25;23(18):3673-7. doi: 10.1093/nar/23.18.3673.
An open reading frame encoding a member of the L16 family of ribosomal proteins is adjacent to the URA7 gene on the left arm of chromosome II in Saccharomyces cerevisiae. The predicted L16-like polypeptide is basic (pl 11.12), contains 232 amino acids (26.52 kDa) and has 36% amino acid sequence identity to E. coli L16. Immunoblot analysis with polyclonal antibodies to the L16-like polypeptide showed specific cross-reaction with a 22,000 Mr mitochondrial polypeptide that co-sediments with the large subunit of the mitochondrial ribosome in sucrose density gradients. The levels of the L16 mRNA and protein varied in response to carbon source. In [rho degree] cells lacking mitochondrial rRNA, the L16 mRNA accumulated at normal levels, but the protein was barely detectable, indicating RNA-dependent accumulation of the L16 protein. Gene disruption experiments demonstrated that the yeast mitochondrial L16 is an essential ribosomal protein in vivo.
在酿酒酵母的II号染色体左臂上，一个编码核糖体蛋白L16家族成员的开放阅读框毗邻URA7基因。预测的类L16多肽呈碱性（pI 11.12），含有232个氨基酸（26.52 kDa），与大肠杆菌L16的氨基酸序列同一性为36%。用针对类L16多肽的多克隆抗体进行免疫印迹分析，结果显示与一个22,000 Mr的线粒体多肽发生特异性交叉反应，该多肽在蔗糖密度梯度中与线粒体核糖体的大亚基共同沉降。L16 mRNA和蛋白质的水平随碳源而变化。在缺乏线粒体rRNA的[rho degree]细胞中，L16 mRNA以正常水平积累，但蛋白质几乎检测不到，这表明L16蛋白的积累依赖于RNA。基因破坏实验表明，酵母线粒体L16在体内是一种必需的核糖体蛋白。
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