• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

使用固定化错配结合蛋白（MutS）进行突变检测。

Mutation detection using immobilized mismatch binding protein (MutS).

作者信息

Wagner R, Debbie P, Radman M

机构信息

Genecheck Inc., Fort Collins, CO 80525, USA.

出版信息

Nucleic Acids Res. 1995 Oct 11;23(19):3944-8. doi: 10.1093/nar/23.19.3944.

DOI:10.1093/nar/23.19.3944
PMID:7479040
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC307314/
Abstract

An accurate and highly sensitive mutation detection assay has been developed. The assay is based on the detection of mispaired and unpaired bases by immobilized mismatch binding protein (Escherichia coli MutS). The assay can detect most mismatches and all single base substitution mutations, as well as small addition or deletion mutations. The assay is simple to use and does not require the use of either radioactivity or gel electrophoresis.

摘要

已经开发出一种准确且高度灵敏的突变检测方法。该方法基于固定化错配结合蛋白（大肠杆菌MutS）对错配和未配对碱基的检测。该方法可以检测大多数错配以及所有单碱基取代突变，还有小的插入或缺失突变。该方法使用简便，无需使用放射性物质或凝胶电泳。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d158/307314/26daf6e71f70/nar00019-0151-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d158/307314/7654f322e980/nar00019-0149-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d158/307314/48db66e06a4a/nar00019-0150-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d158/307314/3d37f8d4ca07/nar00019-0150-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d158/307314/26daf6e71f70/nar00019-0151-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d158/307314/7654f322e980/nar00019-0149-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d158/307314/48db66e06a4a/nar00019-0150-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d158/307314/3d37f8d4ca07/nar00019-0150-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d158/307314/26daf6e71f70/nar00019-0151-a.jpg

相似文献

1
Mutation detection using immobilized mismatch binding protein (MutS).使用固定化错配结合蛋白（MutS）进行突变检测。
Nucleic Acids Res. 1995 Oct 11;23(19):3944-8. doi: 10.1093/nar/23.19.3944.
2
The use of immobilized mismatch binding protein in mutation/SNP detection.固定化错配结合蛋白在突变/单核苷酸多态性检测中的应用。
Methods Mol Biol. 2000;152:159-68. doi: 10.1385/1-59259-068-3:159.
3
Evaluation of MutS as a tool for direct measurement of point mutations in genomic DNA.评估MutS作为直接测量基因组DNA中基因突变工具的性能。
Mutat Res. 1997 Mar 21;374(2):277-85. doi: 10.1016/s0027-5107(96)00245-x.
4
One tube mutation detection using sensitive fluorescent dyeing of MutS protected DNA.使用对MutS保护的DNA进行灵敏荧光染色的单管突变检测。
Nucleic Acids Res. 2000 Apr 15;28(8):E36. doi: 10.1093/nar/28.8.e36.
5
Mutation detection with MutH, MutL, and MutS mismatch repair proteins.利用MutH、MutL和MutS错配修复蛋白进行突变检测。
Proc Natl Acad Sci U S A. 1996 Apr 30;93(9):4374-9. doi: 10.1073/pnas.93.9.4374.
6
Mutation detection by mismatch binding protein, MutS, in amplified DNA: application to the cystic fibrosis gene.通过错配结合蛋白MutS检测扩增DNA中的突变：应用于囊性纤维化基因
Proc Natl Acad Sci U S A. 1994 Mar 29;91(7):2674-8. doi: 10.1073/pnas.91.7.2674.
7
Requirement for Phe36 for DNA binding and mismatch repair by Escherichia coli MutS protein.大肠杆菌MutS蛋白DNA结合及错配修复对苯丙氨酸36的需求。
Nucleic Acids Res. 2000 Sep 15;28(18):3564-9. doi: 10.1093/nar/28.18.3564.
8
MutS recognition: multiple mismatches and sequence context effects.MutS识别：多个错配及序列上下文效应
J Biosci. 2001 Dec;26(5):595-606. doi: 10.1007/BF02704758.
9
Deletion mutation analysis of the mutS gene in Escherichia coli.大肠杆菌中mutS基因的缺失突变分析
J Biol Chem. 1999 Feb 26;274(9):5948-52. doi: 10.1074/jbc.274.9.5948.
10
Crystal structures of mismatch repair protein MutS and its complex with a substrate DNA.错配修复蛋白MutS的晶体结构及其与底物DNA的复合物
Nature. 2000 Oct 12;407(6805):703-10. doi: 10.1038/35037509.

引用本文的文献

1
Enzymatic Methods for Mutation Detection in Cancer Samples and Liquid Biopsies.癌症样本和液体活检测序中基因突变的酶学法检测。
Int J Mol Sci. 2023 Jan 4;24(2):923. doi: 10.3390/ijms24020923.
2
MutS protein-based fiber optic particle plasmon resonance biosensor for detecting single nucleotide polymorphisms.基于 MutS 蛋白的光纤颗粒等离子体共振生物传感器用于检测单核苷酸多态性。
Anal Bioanal Chem. 2021 May;413(12):3329-3337. doi: 10.1007/s00216-021-03271-1. Epub 2021 Mar 13.
3
Towards Automation for Molecular Diagnosis of Cancer.迈向癌症分子诊断自动化

本文引用的文献

1
The rapid detection of unknown mutations in nucleic acids.核酸中未知突变的快速检测。
Nat Genet. 1993 Oct;5(2):111-7. doi: 10.1038/ng1093-111.
2
Screening for mutations by enzyme mismatch cleavage with T4 endonuclease VII.使用T4核酸内切酶VII通过酶错配切割筛选突变。
Proc Natl Acad Sci U S A. 1995 Jan 3;92(1):87-91. doi: 10.1073/pnas.92.1.87.
3
Current methods of mutation detection.当前的突变检测方法。
EJIFCC. 2005 May 17;16(2):66-72. eCollection 2005 May.
4
Diff-seq: A high throughput sequencing-based mismatch detection assay for DNA variant enrichment and discovery.Diff-seq：一种基于高通量测序的错配检测分析方法，用于 DNA 变异富集和发现。
Nucleic Acids Res. 2018 Apr 20;46(7):e42. doi: 10.1093/nar/gky022.
5
A systematic comparison of error correction enzymes by next-generation sequencing.通过下一代测序对纠错酶进行系统比较。
Nucleic Acids Res. 2017 Sep 6;45(15):9206-9217. doi: 10.1093/nar/gkx691.
6
A Novel Technique to Detect EGFR Mutations in Lung Cancer.一种检测肺癌中表皮生长因子受体（EGFR）突变的新技术。
Int J Mol Sci. 2016 May 23;17(5):792. doi: 10.3390/ijms17050792.
7
Comparison of T7E1 and surveyor mismatch cleavage assays to detect mutations triggered by engineered nucleases.比较T7E1和Surveyor错配切割检测法以检测由工程核酸酶引发的突变。
G3 (Bethesda). 2015 Jan 7;5(3):407-15. doi: 10.1534/g3.114.015834.
8
Robust physical methods that enrich genomic regions identical by descent for linkage studies: confirmation of a locus for osteogenesis imperfecta.用于连锁研究的、富集同源基因组区域的强大物理方法：成骨不全症一个基因座的确认
BMC Genet. 2009 Mar 30;10:16. doi: 10.1186/1471-2156-10-16.
9
PCR-based methods for the enrichment of minority alleles and mutations.基于聚合酶链反应的少数等位基因和突变富集方法。
Clin Chem. 2009 Apr;55(4):632-40. doi: 10.1373/clinchem.2008.113035. Epub 2009 Feb 6.
10
A novel method of identifying genetic mutations using an electrochemical DNA array.一种使用电化学DNA阵列识别基因突变的新方法。
Nucleic Acids Res. 2004 Oct 21;32(18):e141. doi: 10.1093/nar/gnh141.
Mutat Res. 1993 Jan;285(1):125-44. doi: 10.1016/0027-5107(93)90060-s.
4
MutS binding protects heteroduplex DNA from exonuclease digestion in vitro: a simple method for detecting mutations.MutS结合可在体外保护异源双链DNA不被核酸外切酶消化：一种检测突变的简单方法。
Nucleic Acids Res. 1994 Jul 11;22(13):2710-1. doi: 10.1093/nar/22.13.2710.
5
Mutation detection by mismatch binding protein, MutS, in amplified DNA: application to the cystic fibrosis gene.通过错配结合蛋白MutS检测扩增DNA中的突变：应用于囊性纤维化基因
Proc Natl Acad Sci U S A. 1994 Mar 29;91(7):2674-8. doi: 10.1073/pnas.91.7.2674.
6
Escherichia coli mutS-encoded protein binds to mismatched DNA base pairs.大肠杆菌mutS编码的蛋白质与错配的DNA碱基对结合。
Proc Natl Acad Sci U S A. 1986 Jul;83(14):5057-61. doi: 10.1073/pnas.83.14.5057.
7
Structures of mismatched base pairs in DNA and their recognition by the Escherichia coli mismatch repair system.DNA中错配碱基对的结构及其被大肠杆菌错配修复系统识别的过程。
EMBO J. 1986 Dec 20;5(13):3697-703. doi: 10.1002/j.1460-2075.1986.tb04702.x.
8
Mispair specificity of methyl-directed DNA mismatch correction in vitro.体外甲基化导向的DNA错配修复的错配特异性
J Biol Chem. 1988 May 15;263(14):6829-35.
9
Mechanisms and biological effects of mismatch repair.错配修复的机制及生物学效应。
Annu Rev Genet. 1991;25:229-53. doi: 10.1146/annurev.ge.25.120191.001305.
10
Repair of DNA heteroduplexes containing small heterologous sequences in Escherichia coli.大肠杆菌中含小异源序列的DNA异源双链体的修复
Proc Natl Acad Sci U S A. 1992 Mar 1;89(5):1730-4. doi: 10.1073/pnas.89.5.1730.