Savva R, Pearl L H
Department of Biochemistry and Molecular Biology, University College London, England.
Proteins. 1995 Jul;22(3):287-9. doi: 10.1002/prot.340220310.
The uracil-DNA glycosylase inhibitory protein (UGI) from the bacteriophage PBS-1 has been cloned and overexpressed. The nucleotide sequence is identical to that for the previously described PBS-2 inhibitor. The recombinant PBS-1 UGI inhibits the uracil-DNA glycosylase from herpes simplex virus type-1 (HSV-1 UDGase), and a complex between the HSV-1 UDGase and PBS-1 UGI has been crystallized. The crystals have unit cell dimensions a = 143.21 A, c = 40.78 A and are in a polar hexagonal space group. There is a single complex in the asymmetric unit with a solvent content of 62% by volume and the crystals diffract to 2.5A on a synchrotron radiation source.
来自噬菌体PBS - 1的尿嘧啶-DNA糖基化酶抑制蛋白（UGI）已被克隆并过量表达。其核苷酸序列与先前描述的PBS - 2抑制剂的序列相同。重组PBS - 1 UGI抑制单纯疱疹病毒1型的尿嘧啶-DNA糖基化酶（HSV - 1 UDGase），并且HSV - 1 UDGase与PBS - 1 UGI之间的复合物已结晶。晶体的晶胞尺寸为a = 143.21 Å，c = 40.78 Å，属于极性六方空间群。不对称单元中有一个单一复合物，溶剂含量为62%（体积），并且这些晶体在同步辐射源上衍射至2.5 Å。
