Allen N D, Logan K, Lally G, Drage D J, Norris M L, Keverne E B
Laboratory of Developmental Genetics and Imprinting, Babraham Institute, Cambridge, United Kingdom.
Proc Natl Acad Sci U S A. 1995 Nov 7;92(23):10782-6. doi: 10.1073/pnas.92.23.10782.
A systematic analysis of parthenogenetic (PG) cell fate within the central nervous system (CNS) was made throughout fetal development and neonatal and adult life. Chimeras were made between PG embryos carrying a ubiquitously expressed lacZ transgene and normal fertilized embryos. After detailed histological analysis, we find that the developmental potential of PG cells is spatially restricted to certain parts of the brain. PG cells are prevalent in telencephalic structures and are largely excluded from diencephalic structures, especially the hypothalamus. These spatial restrictions are established early in development. Behavioral studies with chimeras identified an increase in male aggression when the proportion of PG cells in the brain was high. These studies demonstrate that imprinted genes play key roles in development of the CNS and may be involved in behavior.
在整个胎儿发育、新生儿期及成年期，对中枢神经系统（CNS）内孤雌生殖（PG）细胞的命运进行了系统分析。构建了携带广泛表达的lacZ转基因的PG胚胎与正常受精胚胎之间的嵌合体。经过详细的组织学分析，我们发现PG细胞的发育潜能在空间上局限于大脑的某些部位。PG细胞在端脑结构中普遍存在，而在间脑结构，尤其是下丘脑结构中则基本不存在。这些空间限制在发育早期就已确立。对嵌合体的行为研究表明，当大脑中PG细胞比例较高时，雄性攻击性会增加。这些研究证明，印记基因在中枢神经系统的发育中起关键作用，并且可能与行为有关。
