Li Y, Bjorklund S, Jiang Y W, Kim Y J, Lane W S, Stillman D J, Kornberg R D
Department of Structural Biology, Stanford University School of Medicine, CA 94305, USA.
Proc Natl Acad Sci U S A. 1995 Nov 21;92(24):10864-8. doi: 10.1073/pnas.92.24.10864.
Sin4 and Rgr1 proteins, previously shown by genetic studies to play both positive and negative roles in the transcriptional regulation of many genes, are identified here as components of mediator and RNA polymerase II holoenzyme complexes. Results with Sin4 deletion and Rgr1 truncation strains indicate the association of these proteins in a subcomplex comprising Sin4, Rgr1, Gal11, and a 50-kDa polypeptide. Taken together with the previous genetic evidence, our findings point to a role of the mediator in repression as well as in transcriptional activation.
Sin4和Rgr1蛋白先前经遗传学研究表明在许多基因的转录调控中发挥正负两方面作用，在此被鉴定为中介体和RNA聚合酶II全酶复合物的组分。Sin4缺失和Rgr1截短菌株的实验结果表明这些蛋白在一个包含Sin4、Rgr1、Gal11和一个50 kDa多肽的亚复合物中存在关联。结合先前的遗传学证据，我们的发现表明中介体在基因抑制以及转录激活中均发挥作用。
