Dreyer E B, Leifer D, Heng J E, McConnell J E, Gorla M, Levin L A, Barnstable C J, Lipton S A
Laboratory of Cellular and Molecular Neuroscience, Children's Hospital, Boston, MA, USA.
Proc Natl Acad Sci U S A. 1995 Nov 21;92(24):11195-9. doi: 10.1073/pnas.92.24.11195.
Thy-1, a member of the immunoglobulin superfamily, is one of the most abundant glycoproteins on mammalian neurons. Nevertheless, its role in the peripheral or central nervous system is poorly understood. Certain monoclonal antibodies to Thy-1 promote neurite outgrowth by rodent central nervous system neurons in vitro, suggesting that Thy-1 functions, in part, by modulating neurite outgrowth. We describe a binding site for Thy-1 on astrocytes. This Thy-1-binding protein has been characterized by immunofluroesence with specific anti-idiotype monoclonal antibodies and by three competitive binding assays using (i) anti-idiotype antibodies, (ii) purified Thy-1, and (iii) Thy-1-transfected cells. The Thy-1-binding protein may participate in axonal or dendritic development in the nervous system.
Thy-1是免疫球蛋白超家族的一员，是哺乳动物神经元上最丰富的糖蛋白之一。然而，人们对其在周围或中枢神经系统中的作用了解甚少。某些针对Thy-1的单克隆抗体在体外可促进啮齿动物中枢神经系统神经元的神经突生长，这表明Thy-1部分通过调节神经突生长发挥作用。我们描述了星形胶质细胞上Thy-1的一个结合位点。这种Thy-1结合蛋白已通过使用特异性抗独特型单克隆抗体的免疫荧光以及三种竞争性结合试验进行了表征，这三种试验分别使用（i）抗独特型抗体、（ii）纯化的Thy-1和（iii）Thy-1转染细胞。Thy-1结合蛋白可能参与神经系统中轴突或树突的发育。
