Rocmans P A, Ketelbant-Balasse P, Dumont J E, Neve P
Endocrinology. 1978 Nov;103(5):1834-48. doi: 10.1210/endo-103-5-1834.
Hyperactive dog thyroids were prepared by repeated TSH stimulation in vivo. Butanol-extractable 125I (BE125I) release in vitro from slices and hormonal secretion in vivo in the thyroid vein was enhanced. Apical pseudopods and colloid droplets were very infrequent in such hyperactive thyroids. Moreover, BE125I release was insensitive to cytochalasin B, to inhibitors of microtubules, and to metabolic inhibitors, and decreased only with temperature. Our data demonstrate that hormonal secretion by hyperactive thyroids is not secondary to apical phagocytosis (i.e. macropinocytosis). Micropinocytosis or intraluminal hydrolysis of thyroglobulin are suggested as first step of the secretory process.
通过在体内反复进行促甲状腺激素刺激制备甲状腺功能亢进的犬甲状腺。体外切片中丁醇可提取的125I（BE125I）释放以及甲状腺静脉中体内激素分泌均增强。在这种甲状腺功能亢进的甲状腺中，顶端伪足和胶体小滴非常罕见。此外，BE125I释放对细胞松弛素B、微管抑制剂和代谢抑制剂不敏感，仅随温度降低。我们的数据表明，甲状腺功能亢进的甲状腺激素分泌并非继发于顶端吞噬作用（即巨胞饮作用）。微胞饮作用或甲状腺球蛋白的管腔内水解被认为是分泌过程的第一步。
