Bronson F H, Channing C P
Endocrinology. 1978 Nov;103(5):1894-8. doi: 10.1210/endo-103-5-1894.
Serum levels of FSH in ovariectomized female mice are only partially suppressed by estradiol regardless of the dosage administered. Conversely, within the limits of detection, serum FSH in intact females is totally suppressed by estradiol. Thus, the present experiments used the maximally estrogenized, ovariectomized female as a test system for evaluating other nonestrogenic factors which could depress serum FSH. It was found that a transplanted ovary lowered serum FSH dramatically under such conditions. Of the large number of steroid and pituitary hormones tested, however, only large doses of testosterone propionate yielded any further suppression of serum FSH (24%). Charcoal-extracted follicular fluid (porcine) markedly lowered serum FSH concentrations (50% suppression), while a charcoal-extracted saline homogenate of PMS-treated mouse ovaries also was active in this regard (26% suppression). Finally, charcoal-extracted porcine follicular fluid was administered to nonestrogenized, ovariectomized females, where it both depressed serum FSH and markedly elevated serum LH concentrations. These studies, when viewed in toto, support a contention that follicular fluid contains a nonsteroidal factor which is capable of acting additively with estradiol to regulate FSH secretion. The question of whether this factor also regulates LH secretion in a reciprocal fashion in the mouse requires further work.
在切除卵巢的雌性小鼠中，无论给予何种剂量的雌二醇，其血清促卵泡激素（FSH）水平仅被部分抑制。相反，在可检测范围内，完整雌性小鼠的血清FSH会被雌二醇完全抑制。因此，本实验使用雌激素化程度最高的切除卵巢的雌性小鼠作为测试系统，以评估其他可能降低血清FSH的非雌激素因素。结果发现，在这种情况下，移植的卵巢可显著降低血清FSH水平。然而，在测试的大量类固醇和垂体激素中，只有大剂量的丙酸睾酮能进一步抑制血清FSH（抑制24%）。经活性炭处理的卵泡液（猪）可显著降低血清FSH浓度（抑制50%），而经活性炭处理的经孕马血清促性腺激素（PMS）处理的小鼠卵巢盐水匀浆在这方面也有活性（抑制26%）。最后，将经活性炭处理的猪卵泡液给予未雌激素化的切除卵巢的雌性小鼠，它既能降低血清FSH水平，又能显著提高血清促黄体生成素（LH）浓度。从整体来看，这些研究支持这样一种观点，即卵泡液中含有一种非甾体因子，它能够与雌二醇协同作用来调节FSH的分泌。在小鼠中，这个因子是否也以相反的方式调节LH分泌，还需要进一步研究。
