Tang H, Vasconcelos A C, Berkowitz G A
Plant Science Department, Cook College, New Brunswick, New Jersey, USA.
Plant Physiol. 1995 Sep;109(1):327-30. doi: 10.1104/pp.109.1.327.
Plant K+ channel proteins have been previously characterized as tetramers of membrane-spanning alpha subunit polypeptides. Recent studies have identified a 39-kD, hydrophilic polypeptide that is a structural component of purified animal K+ channel proteins. We have cloned and sequenced an Arabidopsis thaliana cDNA encoding a 38.4-kD polypeptide that has a sequence homologous to the animal K+ channel beta subunit. Southern and northern analyses indicate the presence of a gene encoding this cDNA in the Arabidopsis genome and that its transcription product is present in Arabidopsis cells. To our knowledge, this is the first report to document the presence of K+ channel beta subunits in plants.
植物钾离子通道蛋白先前已被表征为跨膜α亚基多肽的四聚体。最近的研究鉴定出一种39-kD的亲水性多肽,它是纯化的动物钾离子通道蛋白的结构成分。我们已经克隆并测序了拟南芥的一个cDNA,其编码一种38.4-kD的多肽,该多肽具有与动物钾离子通道β亚基同源的序列。Southern和Northern分析表明拟南芥基因组中存在编码该cDNA的基因,并且其转录产物存在于拟南芥细胞中。据我们所知,这是首次报道植物中存在钾离子通道β亚基。