Catt J W, Ryan J P, Pike I L, O'Neill C, Saunders D M
Human Reproduction Unit, Royal North Shore Hospital, St Leonards, NSW, Australia.
Reprod Fertil Dev. 1995;7(2):255-61; discussion 261-2. doi: 10.1071/rd9950255.
The technique of intracytoplasmic sperm injection (ICSI) was first introduced to the Royal North Shore Hospital in April 1993 as part of a controlled study of 100 patient cycles in which sibling oocytes were inseminated by either subzonal insemination (SUZI) or ICSI. This trial showed direct sperm injection to be superior in terms of fertilization. In that study, 58 embryo transfers of 101 ICSI-derived embryos resulted in 10 pregnancies. No miscarriages have occurred and a total of 10 fetal hearts (9.8% per embryo transferred) were detected on ultrasound. There have been 10 deliveries of 10 babies. Since the beginning of 1994, intracytoplasmic injection has been used exclusively for patients requiring micromanipulation to achieve fertilization. There have been 200 patient cycles with 1650 oocytes collected (8.8 oocytes per cycle). Of these oocytes, 1548 were mature (94%) and were subjected to ICSI, and normal fertilization occurred in 874 (56%) of the injected oocytes. The number of oocytes which cleaved and were suitable for fresh transfer or cryopreservation was 818 (94%). There have been 153 fresh embryo transfers of 326 embryos. Twenty-six pregnancies (17% per embryo transfer) have resulted, 22 of which proceeded to ultrasound examination in which 23 fetal hearts were detected (7% per embryo transferred). Three miscarriages have occurred, leaving 19 ongoing pregnancies. There have been 127 cryopreservation procedures involving 492 embryos. To date, there have been 47 embryo thaw cycles, and 93 of the 115 (81%) thawed embryos survived and were transferred. These 47 embryo transfers resulted in 10 pregnancies (21% per embryo transfer), one of which one has miscarried.(ABSTRACT TRUNCATED AT 250 WORDS)
1993年4月，胞浆内单精子注射（ICSI）技术首次被引入皇家北岸医院，作为一项对100个患者周期的对照研究的一部分，在该研究中，同胞卵母细胞通过透明带下授精（SUZI）或ICSI进行授精。该试验表明，直接精子注射在受精方面更具优势。在该研究中，101个ICSI衍生胚胎进行了58次胚胎移植，导致10例妊娠。未发生流产，超声检查共检测到10个胎心（每移植胚胎9.8%）。已分娩10名婴儿。自1994年初以来，胞浆内注射仅用于需要显微操作才能实现受精的患者。共有200个患者周期，收集了1650个卵母细胞（每个周期8.8个卵母细胞）。在这些卵母细胞中，1548个成熟（94%）并接受了ICSI，874个（56%）注射卵母细胞发生了正常受精。分裂且适合新鲜移植或冷冻保存的卵母细胞数量为818个（94%）。对326个胚胎进行了153次新鲜胚胎移植。已导致26例妊娠（每次胚胎移植17%），其中22例进行了超声检查，检测到23个胎心（每次胚胎移植7%）。发生了3次流产，剩下19例正在进行的妊娠。共进行了127次涉及492个胚胎的冷冻保存程序。迄今为止，有47个胚胎解冻周期，115个解冻胚胎中有93个（81%）存活并被移植。这47次胚胎移植导致10例妊娠（每次胚胎移植21%），其中1例流产。（摘要截短于250字）
