飞蝗唾液腺促唾液分泌肽Lom-SG-SASP的分离与鉴定

Isolation and identification of Lom-SG-SASP, a salivation stimulating peptide from the salivary glands of Locusta migratoria.

作者信息

Veelaert D, Schoofs L, Proost P, Van Damme J, Devreese B, Van Beeumen J, De Loof A

机构信息

Zoological Institute, Katholieke Universiteit Leuven, Belgium.

出版信息

Regul Pept. 1995 Jun 27;57(3):221-6. doi: 10.1016/0167-0115(95)00035-A.

DOI:10.1016/0167-0115(95)00035-A

PMID:7480871

Abstract

From a methanolic extract of about 2500 salivary glands of Locusta migratoria a peptide was isolated which stimulates cAMP production in the salivary glands and salivation. Maldi-TOFMS revealed a mass of 1779 Da. The primary structure of the peptide is NH2-EVGDLFKEWLQGNMN-COOH. The peptide is named Locusta migratoria-Salivary Gland-Salivation Stimulating Peptide (Lom-SG-SASP) because of its simulating effect on salivation. Lom-SG-SASP displays no relevant sequence similarities with any other known peptide from vertebrate or invertebrate sources. The effect of synthetic Lom-SG-SASP on cAMP production in the salivary glands and on salivation is discussed.

摘要

从大约2500只飞蝗的唾液腺甲醇提取物中分离出一种肽，该肽可刺激唾液腺中cAMP的产生并促进唾液分泌。基质辅助激光解吸电离飞行时间质谱（Maldi-TOFMS）显示其分子量为1779 Da。该肽的一级结构为NH2-EVGDLFKEWLQGNMN-COOH。由于其对唾液分泌的刺激作用，该肽被命名为飞蝗唾液腺唾液分泌刺激肽（Lom-SG-SASP）。Lom-SG-SASP与脊椎动物或无脊椎动物来源的任何其他已知肽均无相关序列相似性。文中讨论了合成的Lom-SG-SASP对唾液腺中cAMP产生及唾液分泌的影响。