一种来自果蝇的蜕皮抑制素。

An ecdysiostatin from flies.

作者信息

Hua Y J, Koolman J

机构信息

Institute of Physiological Chemistry, Philipps-University, Marburg, Germany.

出版信息

Regul Pept. 1995 Jun 27;57(3):263-71. doi: 10.1016/0167-0115(95)00040-I.

DOI:10.1016/0167-0115(95)00040-I

PMID:7480876

Abstract

A bioassay for compounds with ecdysiostatic activity ('ecdysiostatins') was developed in order to prove the existence of an ecdysiostatin in blowfly larvae. The factor eluted by HPLC like Neb-TMOF (trypsin modulating oostatic factor), a hexapeptide that inhibits ecdysone biosynthesis. The ecdysiostatic activity of Neb-TMOF is specific, related peptides were less active or inactive. TMOF inhibits ecdysone biosynthesis rather than its secretion. It increases the cAMP level of ring glands. Effectors that elevate the intracellular cAMP can mimic the inhibitory effect of the ecdysiostatin. This suggests that cAMP may control steroidogenesis in fly larvae by shutting ecdysone biosynthesis. Thus, the hexapeptide acts as a prothoracicostatic hormone (PTSH) that controls ecdysone biosynthesis.

摘要

为了证明丽蝇幼虫中存在蜕皮抑制素，开发了一种针对具有蜕皮抑制活性的化合物（“蜕皮抑制素”）的生物测定法。该因子通过高效液相色谱法洗脱，类似于Neb-TMOF（胰蛋白酶调节卵生长抑制因子），一种抑制蜕皮激素生物合成的六肽。Neb-TMOF的蜕皮抑制活性具有特异性，相关肽的活性较低或无活性。TMOF抑制蜕皮激素的生物合成而非其分泌。它会增加环腺的cAMP水平。提高细胞内cAMP的效应物可以模拟蜕皮抑制素的抑制作用。这表明cAMP可能通过关闭蜕皮激素生物合成来控制蝇幼虫的类固醇生成。因此，这种六肽作为一种前胸腺抑制激素（PTSH），控制着蜕皮激素的生物合成。