大鼠小肠中胰腺炎相关蛋白I和III基因表达的发育、营养及激素调控

Developmental, nutritional, and hormonal regulation of the pancreatitis-associated protein I and III gene expression in the rat small intestine.

作者信息

Sansonetti A, Romeo H, Berthezene P, Scacchi P, Dusetti N, Keim V, Dagorn J C, Iovanna J L

机构信息

U.315 National Institute of Health and Medical Research, Marseille, France.

出版信息

Scand J Gastroenterol. 1995 Jul;30(7):664-9. doi: 10.3109/00365529509096310.

DOI:10.3109/00365529509096310

PMID:7481529

Abstract

BACKGROUND

The pancreatitis-associated protein (PAP) I and III, but not PAP II, mRNAs are constitutively expressed in the small intestine of rats.

METHODS

We studied expression of both PAP I and PAP III mRNAs during development and on nutritional and hormonal manipulations.

RESULTS

Between day 20 of gestation and day 21 of age, PAP mRNAs could barely be detected. Their concentrations increased dramatically from day 21 to day 45 of age and remained constant thereafter. Rats adapted to a diet with low carbohydrate content showed a significant decrease in PAP mRNA concentrations. Finally, whereas thyroidectomy and ovariectomy induced a decrease in both mRNA concentrations, and adrenalectomy a limited decrease in PAP III mRNA only, diabetes and castration did not alter the expression of either gene.

CONCLUSION

Gene expression of PAP I and III mRNAs is regulated in a coordinate manner in the rat small intestine during development and on nutritional and hormonal manipulations.

摘要

背景

胰腺炎相关蛋白（PAP）I和III的mRNA在大鼠小肠中组成性表达，而PAP II的mRNA则不然。

方法

我们研究了发育过程中以及营养和激素调控情况下PAP I和PAP III mRNA的表达。

结果

在妊娠第20天至出生后第21天期间，几乎检测不到PAP mRNA。从出生后第21天到第45天，它们的浓度急剧增加，此后保持恒定。适应低碳水化合物饮食的大鼠PAP mRNA浓度显著降低。最后，甲状腺切除术和卵巢切除术导致两种mRNA浓度均降低，而肾上腺切除术仅使PAP III mRNA浓度有限降低，糖尿病和阉割则未改变任何一个基因的表达。