Lasserre C, Colnot C, Bréchot C, Poirier F
Unité INSERM 370, CHU Necker and INSERM U 257, Institut Cochin de Génétique Moléculaire, Paris, France.
Am J Pathol. 1999 May;154(5):1601-10. doi: 10.1016/S0002-9440(10)65413-2.
We originally isolated the HIP/PAP gene in a differential screen of a human hepatocellular carcinoma cDNA library. This gene is expressed at high levels in 25% of primary liver cancers but not in nontumorous liver. HIP/PAP belongs to the family of C-type lectins and acts as an adhesion molecule for hepatocytes. In normal adult human tissues, HIP/PAP expression is found in pancreas (exocrine and endocrine cells) and small intestine (Paneth and neuroendocrine cells). In order to gain insight into the possible role of HIP/PAP in vivo, we have investigated the pattern of HIP/PAP expression in the developing postimplantation mouse embryo by in situ hybridization. Detailed analysis of developing mouse embryos revealed that HIP/PAP gene exhibits a restricted expression pattern during development. Thus, HIP/PAP transcripts are first observed within the nervous system from day 14.5 onwards in trigeminal ganglia, dorsal root ganglia, and spinal cord where it appears to be an early specific marker of a subpopulation of motor neurons. At laster stages, HIP/PAP transcripts were detected in intestine and pancreas at day 16.5 but not in embryonic liver. This highly restricted expression pattern suggests that HIP/PAP might participate in neuronal as well as intestinal and pancreatic cell development.
我们最初是在对人肝细胞癌cDNA文库进行差异筛选时分离出HIP/PAP基因的。该基因在25%的原发性肝癌中高水平表达，但在非肿瘤性肝脏中不表达。HIP/PAP属于C型凝集素家族，可作为肝细胞的黏附分子。在正常成人组织中，HIP/PAP表达见于胰腺（外分泌和内分泌细胞）和小肠（潘氏细胞和神经内分泌细胞）。为了深入了解HIP/PAP在体内可能发挥的作用，我们通过原位杂交研究了植入后发育中的小鼠胚胎中HIP/PAP的表达模式。对发育中小鼠胚胎的详细分析表明，HIP/PAP基因在发育过程中呈现出受限的表达模式。因此，从第14.5天起，首先在神经系统的三叉神经节、背根神经节和脊髓中观察到HIP/PAP转录本，在这些部位它似乎是运动神经元亚群的早期特异性标志物。在后期阶段，在第16.5天在肠道和胰腺中检测到HIP/PAP转录本，但在胚胎肝脏中未检测到。这种高度受限的表达模式表明，HIP/PAP可能参与神经元以及肠道和胰腺细胞的发育。
