Linde K, Fthenakis G C, Lippmann R, Kinne J, Abraham A
Institute of Medical Microbiology and Epidemiology, University of Leipzig, Germany.
Vaccine. 1995 Jul;13(10):923-6. doi: 10.1016/0264-410x(95)00010-x.
In each of two experiments, sheep and lambs were vaccinated by a subcutaneous injection of a test vaccine (consisting of a combined serotype 1/2a and serotype 4b live Listeria monocytogenes culture) and challenged 16 days later with a mixture of the homologous wild strains. After challenge, the mortality rate of vaccinated sheep was 28.1% and that of controls 71.9%; that of lambs was 25.0%, although these had been inoculated with the LD70 dose. Furthermore, in each of two field trials in Listeria-infected flocks, primiparous pregnant ewes were vaccinated. In the first field trial, 3 or 110 lambs died of listeriosis of those born of vaccinated (n = 564) or unvaccinated (n = 3345) ewes, respectively. In the second, the perinatal mortality rate of lambs born of vaccinated or unvaccinated ewes was 7.6 or 30.3%, respectively, and the mean birth weight of lambs born of vaccinated or control ewes was 2.2 or 1.8 kg, respectively; the mean milk production of vaccinated ewes was 106 and that of controls 83 l; no Listeria was isolated from milk samples of vaccinated ewes. It is concluded that the vaccine is efficacious for the protection of sheep from listeriosis.
在两项实验中，分别对绵羊和羔羊进行皮下注射试验疫苗（由1/2a血清型和4b血清型单核细胞增生李斯特菌活培养物混合而成）进行免疫接种，并在16天后用同源野生菌株混合物进行攻毒。攻毒后，接种疫苗的绵羊死亡率为28.1%，对照组为71.9%；羔羊死亡率为25.0%，尽管这些羔羊接种的是LD70剂量。此外，在两项针对感染李斯特菌羊群的田间试验中，对初产怀孕母羊进行了免疫接种。在第一项田间试验中，接种疫苗的母羊（n = 564）所产羔羊中有3只死于李斯特菌病，未接种疫苗的母羊（n = 3345）所产羔羊中有110只死于该病。在第二项试验中，接种疫苗或未接种疫苗的母羊所产羔羊的围产期死亡率分别为7.6%和30.3%，接种疫苗的母羊或对照母羊所产羔羊的平均出生体重分别为2.2千克和1.8千克；接种疫苗的母羊平均产奶量为106升，对照组为83升；未从接种疫苗母羊的乳汁样本中分离出李斯特菌。结论是该疫苗对保护绵羊免受李斯特菌病感染有效。
