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与疫情相关的单核细胞增生李斯特菌菌株实验室变体中血清型特异性表面抗原的缺失。

Absence of serotype-specific surface antigen in laboratory variants of epidemic-associated Listeria monocytogenes strains.

作者信息

Cheng Ying, Yue Lili, Elhanafi Driss, Kathariou S

机构信息

North Carolina State University, Department of Food Science, Campus Box 2764, Raleigh, NC 27695-2764, USA.

出版信息

Appl Environ Microbiol. 2007 Oct;73(19):6313-6. doi: 10.1128/AEM.00473-07. Epub 2007 Aug 3.

Abstract

Variants that lacked reactivity with the serotype 4b-specific monoclonal antibody c74.22 and that lost susceptibility to certain Listeria- or serotype 4b-specific phages were identified in the course of genetic studies with serotype 4b Listeria monocytogenes strains H7550 and F2381L (epidemic clones I and II, respectively). Our findings suggest that such variants can become inadvertently established under laboratory conditions and suggest caution in work involving serotype 4b strains and genetic constructs thereof.

摘要

在对血清型4b单核细胞增生李斯特菌菌株H7550和F2381L(分别为流行克隆I和II)进行基因研究的过程中,鉴定出了与血清型4b特异性单克隆抗体c74.22缺乏反应性且对某些李斯特菌或血清型4b特异性噬菌体失去敏感性的变体。我们的研究结果表明,此类变体可能会在实验室条件下无意中出现,并提示在涉及血清型4b菌株及其基因构建体的工作中要谨慎行事。

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