Cheng Ying, Yue Lili, Elhanafi Driss, Kathariou S
North Carolina State University, Department of Food Science, Campus Box 2764, Raleigh, NC 27695-2764, USA.
Appl Environ Microbiol. 2007 Oct;73(19):6313-6. doi: 10.1128/AEM.00473-07. Epub 2007 Aug 3.
Variants that lacked reactivity with the serotype 4b-specific monoclonal antibody c74.22 and that lost susceptibility to certain Listeria- or serotype 4b-specific phages were identified in the course of genetic studies with serotype 4b Listeria monocytogenes strains H7550 and F2381L (epidemic clones I and II, respectively). Our findings suggest that such variants can become inadvertently established under laboratory conditions and suggest caution in work involving serotype 4b strains and genetic constructs thereof.
在对血清型4b单核细胞增生李斯特菌菌株H7550和F2381L(分别为流行克隆I和II)进行基因研究的过程中,鉴定出了与血清型4b特异性单克隆抗体c74.22缺乏反应性且对某些李斯特菌或血清型4b特异性噬菌体失去敏感性的变体。我们的研究结果表明,此类变体可能会在实验室条件下无意中出现,并提示在涉及血清型4b菌株及其基因构建体的工作中要谨慎行事。