Bercovich Z, Eger A, Dekker T, Haagsma J
Department of Bacteriology, Central Veterinary Institute, Lelystad, The Netherlands.
Zentralbl Veterinarmed B. 1995 Mar;42(1):19-27. doi: 10.1111/j.1439-0450.1995.tb00677.x.
A study was conducted to evaluate the biological activity of Brucella allergens extracted with hydrochloride or trichloroacetic acid. Smooth and mucoid Brucella abortus cells and the medium in which brucellae were propagated were used to prepare the allergens. The biological activity of the allergens was estimated in guinea-pigs sensitized with Brucella abortus strain 544. The guinea-pigs were intradermally injected with several allergen dilutions. The dilutions were coded and randomized for site of injection so that none of the dilution was injected twice on the same site. Variance analysis using incomplete Latin square was used for the statistical calculation of the results. The calculated biological activity of the allergens was compared with the biological activity of a 'standard' allergen that has proved effective in detecting cattle brucellosis. The skin erythema diameter was best when recorded 32 h after allergen injection. Statistical analysis of the skin erythema diameters showed a great variation in biological activity (12-105%) between the allergens. Only the allergen extracted from the medium in which a mucoid Brucella strain was propagated was as potent as the standard. The use of the incomplete Latin square for variance analysis resulted in the estimation of the biological activity of nine batches of allergen in only 27 guinea-pigs.
开展了一项研究，以评估用盐酸或三氯乙酸提取的布鲁氏菌变应原的生物活性。使用光滑型和黏液型流产布鲁氏菌细胞以及布鲁氏菌繁殖所用的培养基来制备变应原。在用流产布鲁氏菌544菌株致敏的豚鼠中评估变应原的生物活性。给豚鼠皮内注射几种变应原稀释液。对稀释液进行编码并随机安排注射部位，以便同一部位不会注射两次相同的稀释液。使用不完全拉丁方进行方差分析来对结果进行统计计算。将计算得出的变应原生物活性与已证明在检测牛布鲁氏菌病方面有效的“标准”变应原的生物活性进行比较。变应原注射32小时后记录皮肤红斑直径最为适宜。对皮肤红斑直径的统计分析表明，变应原之间的生物活性存在很大差异（12% - 105%）。只有从黏液型布鲁氏菌菌株繁殖所用的培养基中提取的变应原与标准变应原效力相当。使用不完全拉丁方进行方差分析，仅用27只豚鼠就完成了九批变应原生物活性的评估。
