Peters M, Pohlenz J, Jaton K, Ninet B, Bille J
Institute of Microbiology and Infectious Diseases of Animals, School of Veterinary Medicine, Hannover, Germany.
Zentralbl Veterinarmed B. 1995 Apr;42(2):84-8. doi: 10.1111/j.1439-0450.1995.tb00686.x.
A total of 14 cerebrospinal fluid (CSF) samples from ruminants clinically suspected of suffering from listeric encephalitis were examined by polymerase chain reaction (PCR) for the detection of Listeria monocytogenes (L. m.). Of these samples, 11 were examined bacteriologically. Although the clinical diagnosis was confirmed in eight of 11 ruminants by histological and/or bacteriological examination of the brains, L. m. was only detected in one of the CSF samples using PCR, and in none by culture. The PCR-positive CSF sample was obtained from a sheep which had been treated with antibiotics prior to CSF sampling. From these findings, it was concluded that L. m. only occasionally gains access to the meningoventricular system in the course of listeric encephalitis of ruminants and that a reliable aetiological in vivo diagnosis of listeric encephalitis generally cannot be based on the detection of L. m. in the CSF of affected ruminants.
对14份临床怀疑患有李斯特菌性脑炎的反刍动物脑脊液（CSF）样本进行了聚合酶链反应（PCR）检测，以检测单核细胞增生李斯特菌（L. m.）。其中11份样本进行了细菌学检查。尽管通过对大脑进行组织学和/或细菌学检查在11只反刍动物中的8只中确诊了临床诊断，但使用PCR仅在一份脑脊液样本中检测到L. m.，而通过培养未检测到。PCR阳性的脑脊液样本取自一只在采集脑脊液样本前已接受抗生素治疗的绵羊。从这些结果可以得出结论，在反刍动物李斯特菌性脑炎过程中，L. m.仅偶尔进入脑脊膜脑室系统，并且李斯特菌性脑炎可靠的体内病因诊断通常不能基于在受影响反刍动物的脑脊液中检测到L. m.。
