Aarestrup F M, Wegener H C, Rosdahl V T
Danish Veterinary Laboratory, Copenhagen.
Acta Vet Scand. 1995;36(2):237-43. doi: 10.1186/BF03547692.
Fifty-two strains of S. aureus isolated from cases of bovine subclinical mastitis in 52 different dairy herds in Denmark, in the periods 1952 to 1956 and 1992, were compared with regard to their phage- and EcoRI ribotypes. Furthermore, susceptibility to penicillin and production of fibrinolysin were used as additional phenotypic markers. Forty-nine strains (94%) could be separated into 12 phage types. Ribotyping assigned the 52 strains to 21 different types. Both methods showed that 57% of the 1950's strains and between 38-45% of the 1992 strains belonged to 3 dominating types. The remaining strains were placed by ribotyping in 8 types occurring among the 1952-1956 strains and 10 types occurring among the 1992 strains. In 87% of the strains the results of the 2 typing methods were in accordance. However, 7 strains gave different results by the 2 methods including 2 strains with major differences. Penicillin resistance only occurred in a single genotype from the 1950's compared to 6 different genotypes among the 1992 strains.
对1952年至1956年以及1992年期间从丹麦52个不同奶牛场的牛亚临床乳腺炎病例中分离出的52株金黄色葡萄球菌，就其噬菌体和EcoRI核糖型进行了比较。此外，还将对青霉素的敏感性和纤维蛋白溶酶的产生用作额外的表型标记。49株（94%）菌株可分为12种噬菌体类型。核糖分型将这52株菌株分为21种不同类型。两种方法均显示，20世纪50年代的菌株中有57%以及1992年的菌株中有38%-45%属于3种主要类型。其余菌株通过核糖分型被分为在1952-1956年的菌株中出现的8种类型以及在1992年的菌株中出现的10种类型。在87%的菌株中，两种分型方法的结果一致。然而，有7株菌株通过两种方法得出了不同结果，其中包括2株有重大差异的菌株。与1992年菌株中的6种不同基因型相比，青霉素抗性仅在20世纪50年代的单一基因型中出现。
