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南美洲和中美洲白纹伊蚊种群核糖体DNA基因间隔区的变异

Variation in ribosomal DNA intergenic spacers among populations of Anopheles albimanus in South and Central America.

作者信息

De Merida A M, De Mata M P, Molina E, Porter C H, Black W C

机构信息

Universidad del Valle de Guatemala, Guatemala City, Guatemala.

出版信息

Am J Trop Med Hyg. 1995 Nov;53(5):469-77. doi: 10.4269/ajtmh.1995.53.469.

DOI:10.4269/ajtmh.1995.53.469
PMID:7485704
Abstract

Variation in the length and copy number of intergenic spacers (IGS) of nuclear ribosomal DNA were examined to test for genetic differentiation among Anopheles albimanus populations. Extensive collections were made in Guatemala but populations were also sampled over a large range of its distribution in Central and South America. Discriminant analysis of IGS patterns in individual mosquitoes indicated that populations generally had unique sets of IGS length variants. The IGS patterns from populations on the Pacific side of Central America were distinct from those on the Atlantic side or from South America. Cluster analysis indicated a similar trend. The IGS diversity in Central America was 50% greater than in South America. These results suggest that barriers to gene flow exist among Atlantic and Pacific coast populations of An. albimanus. No gene flow barriers were detected among populations from Colombia, Ecuador, Peru, and Venezuela.

摘要

研究了核糖体DNA基因间隔区(IGS)长度和拷贝数的变异,以检测白纹伊蚊种群之间的遗传分化。在危地马拉进行了大量采集,但也在中美洲和南美洲其分布的广泛区域对种群进行了采样。对单个蚊子的IGS模式进行判别分析表明,种群通常具有独特的IGS长度变异集。中美洲太平洋一侧种群的IGS模式与大西洋一侧或南美洲的种群不同。聚类分析显示了类似的趋势。中美洲的IGS多样性比南美洲高50%。这些结果表明,白纹伊蚊大西洋和太平洋沿岸种群之间存在基因流动障碍。在哥伦比亚、厄瓜多尔、秘鲁和委内瑞拉的种群之间未检测到基因流动障碍。

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